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    Primers for PCR amplification comprising a basic parts within the primer sequences
    1.
    发明授权
    Primers for PCR amplification comprising a basic parts within the primer sequences 有权
    用于PCR扩增的引物，其包含引物序列内的基本部分

    公开(公告)号：US08513399B2

    公开(公告)日：2013-08-20

    申请号：US12681754

    申请日：2008-10-02

    申请人： Hyun Bae Kim ,  Seong Youl Kim ,  Jun Mo Gil ,  Hae Joon Park ,  Han Oh Park

    发明人： Hyun Bae Kim ,  Seong Youl Kim ,  Jun Mo Gil ,  Hae Joon Park ,  Han Oh Park

    IPC分类号： C07H21/04 ,  C07H19/04 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/686 ,  C12Q2525/119

    摘要： The present invention relates to primers for PCR amplification comprising abasic parts within the primer sequences and a method for PCR amplification using the same. More precisely, the present invention relates to primers capable of amplifying different templates and having abasic parts complementary to mutated site or polymorphic site of template DNA and a method for PCR amplification comprising the steps of mixing the composition for PCR amplification comprising the primers with nucleic acid template; and performing PCR with the mixture. The primers for PCR amplification of the present invention contain abasic parts not having specific coding information in their nucleotide sequences, so that they can amplify different templates having mutated sites at the same time.

    摘要翻译： 本发明涉及用于PCR扩增的引物，其包括引物序列内的无碱部分和使用其的PCR扩增方法。 更确切地说，本发明涉及能够扩增不同模板并具有与模板DNA的突变位点或多态位点互补的脱碱基的引物，以及用于PCR扩增的方法，其包括将包含引物的PCR扩增组合物与核酸 模板; 并用混合物进行PCR。 用于本发明的PCR扩增的引物含有在其核苷酸序列中不具有特定编码信息的脱碱基，从而可以同时扩增具有突变位点的不同模板。

    PRIMERS FOR PCR AMPLIFICATION COMPRISING A BASIC PARTS WITHIN THE PRIMER SEQUENCES
    2.
    发明申请
    PRIMERS FOR PCR AMPLIFICATION COMPRISING A BASIC PARTS WITHIN THE PRIMER SEQUENCES 有权
    用于在扩展序列中包含基本部分的PCR扩增的引物

    公开(公告)号：US20110008845A1

    公开(公告)日：2011-01-13

    申请号：US12681754

    申请日：2008-10-02

    申请人： Hyun Bae Kim ,  Seong Youl Kim ,  Jun Mo Gil ,  Hae Joon Park ,  Han Oh Park

    发明人： Hyun Bae Kim ,  Seong Youl Kim ,  Jun Mo Gil ,  Hae Joon Park ,  Han Oh Park

    IPC分类号： C12P19/34 ,  C07H21/00 ,  C12N9/12

    CPC分类号： C12Q1/686 ,  C12Q2525/119

    摘要： The present invention relates to primers for PCR amplification comprising abasic parts within the primer sequences and a method for PCR amplification using the same. More precisely, the present invention relates to primers capable of amplifying different templates and having abasic parts complementary to mutated site or polymorphic site of template DNA and a method for PCR amplification comprising the steps of mixing the composition for PCR amplification comprising the primers with nucleic acid template; and performing PCR with the mixture. The primers for PCR amplification of the present invention contain abasic parts not having specific coding information in their nucleotide sequences, so that they can amplify different templates having mutated sites at the same time.

    摘要翻译： 本发明涉及用于PCR扩增的引物，其包括引物序列内的无碱部分和使用其的PCR扩增方法。 更确切地说，本发明涉及能够扩增不同模板并具有与模板DNA的突变位点或多态位点互补的脱碱基的引物，以及用于PCR扩增的方法，其包括将包含引物的PCR扩增组合物与核酸 模板; 并用混合物进行PCR。 用于本发明的PCR扩增的引物含有在其核苷酸序列中不具有特定编码信息的脱碱基，从而可以同时扩增具有突变位点的不同模板。

    DRIED COMPOSITION FOR HOT-START PCR WITH LONG-TERM STABILITY
    3.
    发明申请
    DRIED COMPOSITION FOR HOT-START PCR WITH LONG-TERM STABILITY 有权
    用于具有长期稳定性的热启动PCR的干燥组合物

    公开(公告)号：US20100209973A1

    公开(公告)日：2010-08-19

    申请号：US12682456

    申请日：2008-10-28

    申请人： Seong-Youl Kim ,  Hyun Bae Kim ,  Hae-Joon Park ,  Han Oh Park

    发明人： Seong-Youl Kim ,  Hyun Bae Kim ,  Hae-Joon Park ,  Han Oh Park

    IPC分类号： C12P19/34 ,  C12N9/14

    CPC分类号： C12Q1/686 ,  C12Q2549/101 ,  C12Q2527/125

    摘要： The present invention relates to a dried composition for hot-start PCR, more precisely a dried composition for hot-start PCR with improved stability and long-term storagability which is characteristically prepared by the steps of preparing a reaction mixture by mixing an aqueous solution containing reaction buffer, MgCl2, 4 types of dNTPs, DNA polymerase with pyrophosphate and pyrophosphatase in a reaction tube; and drying the reaction mixture prepared above, a preparation method of the same and a method for amplifying nucleic acid using the same. The dried composition for hot-start PCR is added with pyrophosphate and pyrophosphatase together before drying, so that it can have improved stability and long-term storagability as well as convenience in use, compared with the conventional compositions for hot-start PCR. Therefore, this composition can be effectively used for hot-start PCR, multiplex PCR or real-time quantitative PCR.

    摘要翻译： 本发明涉及一种用于热启动PCR的干燥组合物，更准确地说是一种用于热启动PCR的干燥组合物，其具有改进的稳定性和长期储存性，其特征在于通过以下步骤制备反应混合物：将含有 反应缓冲液，MgCl 2，4种dNTP，反应管中含有焦磷酸的DNA聚合酶和焦磷酸酶; 并干燥上述制备的反应混合物，其制备方法和使用其制备用于扩增核酸的方法。 用于热启动PCR的干燥组合物在干燥之前加入焦磷酸盐和焦磷酸酶，与常规的热启动组合物相比，其可以具有改善的稳定性和长期储存性以及使用方便性。 因此，该组合物可有效用于热启动PCR，多重PCR或实时定量PCR。

    Dried composition for hot-start PCR with long-term stability
    4.
    发明授权
    Dried composition for hot-start PCR with long-term stability 有权
    用于热启动PCR的干组合物具有长期稳定性

    公开(公告)号：US09034603B2

    公开(公告)日：2015-05-19

    申请号：US12682456

    申请日：2008-10-28

    申请人： Seong-Youl Kim ,  Hyun Bae Kim ,  Hae-Joon Park ,  Han Oh Park

    发明人： Seong-Youl Kim ,  Hyun Bae Kim ,  Hae-Joon Park ,  Han Oh Park

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686 ,  C12Q2549/101 ,  C12Q2527/125

    摘要： The present invention relates to a dried composition for hot-start PCR, more precisely a dried composition for hot-start PCR with improved stability and long-term storagability which is characteristically prepared by the steps of preparing a reaction mixture by mixing an aqueous solution containing reaction buffer, MgCl2, 4 types of dNTPs, DNA polymerase with pyrophosphate and pyrophosphatase in a reaction tube; and drying the reaction mixture prepared above, a preparation method of the same and a method for amplifying nucleic acid using the same. The dried composition for hot-start PCR is added with pyrophosphate and pyrophosphatase together before drying, so that it can have improved stability and long-term storagability as well as convenience in use, compared with the conventional compositions for hot-start PCR. Therefore, this composition can be effectively used for hot-start PCR, multiplex PCR or real-time quantitative PCR.

    摘要翻译： 本发明涉及一种用于热启动PCR的干燥组合物，更准确地说是一种用于热启动PCR的干燥组合物，其具有改进的稳定性和长期储存性，其特征在于通过以下步骤制备反应混合物：将含有 反应缓冲液，MgCl 2，4种dNTP，反应管中含有焦磷酸的DNA聚合酶和焦磷酸酶; 并干燥上述制备的反应混合物，其制备方法和使用其制备用于扩增核酸的方法。 用于热启动PCR的干燥组合物在干燥之前加入焦磷酸盐和焦磷酸酶，与常规的热启动组合物相比，其可以具有改善的稳定性和长期储存性以及使用方便性。 因此，该组合物可有效用于热启动PCR，多重PCR或实时定量PCR。

    Cyclic reverse transcription method
    6.
    发明授权
    Cyclic reverse transcription method 有权
    循环逆转录法

    公开(公告)号：US08058007B2

    公开(公告)日：2011-11-15

    申请号：US12531778

    申请日：2008-03-19

    申请人： Hui Jeong Jeong ,  Min-Jung Kim ,  Hae-Joon Park ,  Han Oh Park

    发明人： Hui Jeong Jeong ,  Min-Jung Kim ,  Hae-Joon Park ,  Han Oh Park

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6846 ,  C12Q2537/155 ,  C12Q2527/101 ,  C12Q2521/107

    摘要： Disclosed is a cyclic reverse transcription method, which comprises performing reverse transcription reaction in one or more cycles, each cycle comprising the steps of: (i) performing reaction with a reverse transcription reaction solution comprising template RNA, RNA-dependent DNA polymerase, a reaction buffer, primers for reverse transcription and dNTPs, and optionally, a stabilizer, at 10° C. to 40° C. and, (ii) performing reaction with the resultant reaction solution at 42° to 55° C.

    摘要翻译： 公开了一种循环逆转录方法，其包括在一个或多个循环中进行逆转录反应，每个循环包括以下步骤：（i）与包含模板RNA，RNA依赖性DNA聚合酶的反转录反应溶液，反应 缓冲液，用于逆转录的引物和dNTP，以及任选的稳定剂，在10℃至40℃下，和（ii）在42℃至55℃下与所得反应溶液进行反应。

    CYCLIC REVERSE TRANSCRIPTION METHOD
    7.
    发明申请
    CYCLIC REVERSE TRANSCRIPTION METHOD 有权
    循环逆转录方法

    公开(公告)号：US20100221786A1

    公开(公告)日：2010-09-02

    申请号：US12531778

    申请日：2008-03-19

    申请人： Hui Jeong Jeong ,  Min-Jung Kim ,  Hae-Joon Park ,  Han Oh Park

    发明人： Hui Jeong Jeong ,  Min-Jung Kim ,  Hae-Joon Park ,  Han Oh Park

    IPC分类号： C12P19/34

    CPC分类号： C12Q1/6846 ,  C12Q2537/155 ,  C12Q2527/101 ,  C12Q2521/107

    摘要： Disclosed is a cyclic reverse transcription method, which comprises performing reverse transcription reaction in one or more cycles, each cycle comprising the steps of: (i) performing reaction with a reverse transcription reaction solution comprising template RNA, RNA-dependent DNA polymerase, a reaction buffer, primers for reverse transcription and dNTPs, and optionally, a stabilizer, at 10° C. to 40° G and, (ii) performing reaction with the resultant reaction solution at 42° to 55° C.

    摘要翻译： 公开了一种循环逆转录方法，其包括在一个或多个循环中进行逆转录反应，每个循环包括以下步骤：（i）与包含模板RNA，RNA依赖性DNA聚合酶的反转录反应溶液，反应 缓冲液，用于逆转录的引物和dNTP，以及任选的稳定剂，在10℃至40℃下，和（ii）在42℃至55℃下与所得反应溶液进行反应。

    Method for isolating a nucleic acid using particulate matter and a composition therefor
    8.
    发明授权
    Method for isolating a nucleic acid using particulate matter and a composition therefor 有权
    使用颗粒物分离核酸的方法及其组合物

    公开(公告)号：US08530639B2

    公开(公告)日：2013-09-10

    申请号：US12448134

    申请日：2007-12-11

    申请人： Jong-Hoon Kim ,  Min Kim ,  Hae-Joon Park ,  Han Oh Park

    发明人： Jong-Hoon Kim ,  Min Kim ,  Hae-Joon Park ,  Han Oh Park

    IPC分类号： C07H21/00

    CPC分类号： C07H21/00 ,  C12N15/1003

    摘要： A method for isolating a nucleic acid from a biological sample includes applying particulate matter to promote co-aggregation and co-precipitation of insoluble aggregate by directly adding to the biological sample, adding to the biological sample in admixture with a cell lysis buffer, adding to the biological sample treated with a cell lysis buffer, adding to cell lysates in admixture with a buffer for forming denatured protein aggregate; or adding to cell lysates comprising the formed denatured protein aggregate. The particulate matter is selected from the group consisting of a material formed from an element of Ag, Fe, Ti, Al, Sn, Si, Cu, Mo, Ni, W or Zn, an oxide, a carbide, a nitride, a boride and a silicide thereof, and a mixture thereof, a polymer selected from PMMA (Poly Methyl MethAcrylate), polyethylene or polyurethane; and a mixture thereof. The insoluble aggregate comprises denatured protein aggregate and cell debris.

    摘要翻译： 从生物样品中分离核酸的方法包括通过直接添加到生物样品中来加入颗粒物质以促进不溶性聚集体的共聚和共沉淀，将生物样品与细胞裂解缓冲液混合，加入到 用细胞裂解缓冲液处理生物样品，加入细胞裂解物与缓冲液混合以形成变性蛋白质聚集体; 或加入包含形成的变性蛋白质聚集体的细胞裂解物。 颗粒物质选自由Ag，Fe，Ti，Al，Sn，Si，Cu，Mo，Ni，W或Zn的元素形成的材料，氧化物，碳化物，氮化物，硼化物 及其硅化物及其混合物，选自PMMA（聚甲基丙烯酸甲酯），聚乙烯或聚氨酯的聚合物; 及其混合物。 不溶性聚集体包含变性蛋白质聚集体和细胞碎片。

    METHOD FOR ISOLATING A NUCLEIC ACID USING PARTICULATE MATTER AND A COMPOSITION THEREFOR
    9.
    发明申请
    METHOD FOR ISOLATING A NUCLEIC ACID USING PARTICULATE MATTER AND A COMPOSITION THEREFOR 有权
    使用颗粒物分解核酸的方法及其组合物

    公开(公告)号：US20100197903A1

    公开(公告)日：2010-08-05

    申请号：US12448134

    申请日：2007-12-11

    申请人： Jong-Hoon Kim ,  Min Kim ,  Hae-Joon Park ,  Han Oh Park

    发明人： Jong-Hoon Kim ,  Min Kim ,  Hae-Joon Park ,  Han Oh Park

    IPC分类号： C07H21/00 ,  B01J20/26 ,  C09K3/00

    CPC分类号： C07H21/00 ,  C12N15/1003

    摘要： Disclosed are a method for isolating a nucleic acid using particulate matter and a composition therefor. The method comprises essentially the steps of adding a lysis buffer and a neutralization buffer to a biological sample sequentially, and centrifuging cell lysates for at least 10 minutes to separate a solution comprising the nucleic acid and insoluble aggregate comprising denatured protein aggregate and cell debris. The particulate matter is added to and participates in co-aggregation and co-precipitation of denatured protein aggregate and cell debris, and co-aggregates and co-precipitates denatured protein aggregate and cell debris within a much shortened time, thereby to shorten the time and to increase the yield for the isolation of a nucleic acid, compared with conventional methods.

    摘要翻译： 公开了使用颗粒物质及其组合物分离核酸的方法。 该方法基本上包括以下步骤：依次向生物样品中加入裂解缓冲液和中和缓冲液，并离心细胞裂解物至少10分钟以分离包含核酸的溶液和包含变性蛋白质聚集体和细胞碎片的不溶性骨架。 添加颗粒物并参与变性蛋白质聚集体和细胞碎片的共聚和共沉淀，并在更短的时间内共同聚集和共沉淀变性蛋白质聚集体和细胞碎片，从而缩短时间和 以提高分离核酸的产量，与常规方法相比。

    Detection Method of Dna Amplification Using Probe Labeled With Intercalating Dye
    10.
    发明申请
    Detection Method of Dna Amplification Using Probe Labeled With Intercalating Dye 审中-公开
    用插入染料标记的探针的Dna扩增检测方法

    公开(公告)号：US20080220415A1

    公开(公告)日：2008-09-11

    申请号：US10593900

    申请日：2005-03-25

    申请人： Han Oh Park ,  Hyun-Bae Kim ,  Sung-Min Chi

    发明人： Han Oh Park ,  Hyun-Bae Kim ,  Sung-Min Chi

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6844 ,  C12Q1/686 ,  C12Q2563/173 ,  C12Q2563/107 ,  C12Q2561/113

    摘要： The present invention relates to a detection method of nucleic acid amplification using probe labeled with intercalating dye. More particularly, the present invention is directed to a real-time detection method of nucleic acid amplification, comprising the steps of i) producing an aqueous buffer which contains a nucleic acid, a pair of primers for amplification of said nucleic acid, a fluorescent probe wherein a fluorescent dye of which intensity of fluorescence is varied when the dye is intercalated into a double-stranded nucleic acid, is connected with an oligonucleotide of which base sequence is complementary with at least a part of said nucleic acid, four (4) kinds of nucleotides and DNA polymerase; ii) denaturing said doublestranded nucleic acid into single strands by heating the aqueous buffer prepared in step i) up to 931 C to 96 C; iii) annealing said pair of primers with said single strand by cooling the solution obtained in step ii) up to 50 C. to 571 C; iv) replicating said single-stranded nucleic acid by heating the solution obtained from step iii) up to 701 C to 74° C.; v) denaturing said replicated nucleic add into single strands by heating the solution obtained in step iv) up to 931 C to 961 C; vi) annealing said fluorescent probe with said single-stranded nucleic acid by cooling the solution obtained in step v up to 501 C to 57 C; vii) measuring an intensity of the fluorescence emitted from the solution obtained in step vi); and viii) repeating more than one steps iv) through vii).

    摘要翻译： 本发明涉及使用用插层染料标记的探针的核酸扩增检测方法。 更具体地，本发明涉及核酸扩增的实时检测方法，包括以下步骤：i）产生含有核酸的水性缓冲液，用于扩增所述核酸的一对引物，荧光探针 其中当染料插入双链核酸时荧光强度变化的荧光染料与其碱基序列与所述核酸的至少一部分互补的寡核苷酸连接，四（4）种 的核苷酸和DNA聚合酶; ii）通过加热步骤i）中制备的含水缓冲液至931℃至96℃，将所述双链核酸变性为单链; iii）通过将步骤ii）中获得的溶液冷却至50℃至571℃，用所述单链退火所述一对引物; iv）通过将从步骤iii）获得的溶液加热至701℃至74℃来复制所述单链核酸; v）通过将步骤iv）中获得的溶液加热至931℃至961℃，将所述复制的核酸添加到单链中; vi）通过将步骤v中获得的溶液冷却至501℃至57℃，用所述单链核酸退火所述荧光探针; vii）测量从步骤vi）中获得的溶液发射的荧光的强度; 和viii）重复多于一个步骤iv）至vii）。