A system and method for analyzing tissue healing may include capturing an image including a tissue site. A clinician may be enabled to define multiple regions of the image of the tissue site, where at least two of the regions of the image define tissue types at different stages of tissue healing. An area may be calculated of each of the at least two regions of the image defining tissue type at different stages of tissue healing. The calculated areas may be displayed to a clinician. In one embodiment, an image color adjustment may be determined by adjusting the image of the reference color marker to the predetermined color. The image of the tissue site may be normalized by applying the image color adjustment to generate a normalized image.