- 专利标题： Process for improved protein expression by strain engineering
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申请号： US11189375申请日： 2005-07-26
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公开(公告)号： US08603824B2公开(公告)日： 2013-12-10
- 发明人: Thomas M. Ramseier , Hongfan Jin , Charles H. Squires
- 申请人： Thomas M. Ramseier , Hongfan Jin , Charles H. Squires
- 申请人地址： US CA San Diego
- 专利权人： Pfenex, Inc.
- 当前专利权人： Pfenex, Inc.
- 当前专利权人地址： US CA San Diego
- 代理机构： Wilson, Sonsini, Goodrich & Rosati
- 主分类号： C12N15/67
- IPC分类号： C12N15/67 ; C12N15/63 ; C12N15/64 ; C12N15/65 ; C12N15/52
摘要：
This invention is a process for improving the production levels of recombinant proteins or peptides or improving the level of active recombinant proteins or peptides expressed in host cells. The invention is a process of comparing two genetic profiles of a cell that expresses a recombinant protein and modifying the cell to change the expression of a gene product that is upregulated in response to the recombinant protein expression. The process can improve protein production or can improve protein quality, for example, by increasing solubility of a recombinant protein.