-
公开(公告)号：US08603824B2
公开(公告)日：2013-12-10
申请号：US11189375
申请日：2005-07-26
摘要： This invention is a process for improving the production levels of recombinant proteins or peptides or improving the level of active recombinant proteins or peptides expressed in host cells. The invention is a process of comparing two genetic profiles of a cell that expresses a recombinant protein and modifying the cell to change the expression of a gene product that is upregulated in response to the recombinant protein expression. The process can improve protein production or can improve protein quality, for example, by increasing solubility of a recombinant protein.
-
2.发明授权Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins 有权用于快速筛选微生物宿主以鉴定具有提高异源蛋白质表达的产量和/或质量的某些菌株的方法公开(公告)号：US09394571B2
公开(公告)日：2016-07-19
申请号：US12109554
申请日：2008-04-25
申请人： Thomas M. Ramseier , Russell J. Coleman , Jane C. Schneider , Charles D. Hershberger , Diane M. Retallack , Charles H. Squires
发明人： Thomas M. Ramseier , Russell J. Coleman , Jane C. Schneider , Charles D. Hershberger , Diane M. Retallack , Charles H. Squires
IPC分类号： G01N33/554 , C12N1/20 , C12N15/00 , C12P21/04 , C12P21/06 , C07H21/04 , C12Q1/68 , C12N15/78
摘要： The present invention provides an array for rapidly identifying a host cell population capable of producing a heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest including therapeutic proteins, hormones, growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.
摘要翻译： 本发明提供了一种用于快速鉴定能够产生具有改善的产量和/或质量的异源蛋白质的宿主细胞群的阵列。 该阵列包含已被遗传修饰的一个或多个宿主细胞群，以增加涉及蛋白质产生的一个或多个靶基因的表达，减少参与蛋白质降解的一个或多个靶基因的表达，或两者。 阵列中的一个或多个菌株可以在周质隔室中表达感兴趣的异源蛋白质，或者通过外细胞壁在细胞外分泌异源蛋白质。 菌株阵列可用于筛选任何目的蛋白质的表达，包括治疗性蛋白质，激素，生长因子，细胞外受体或配体，蛋白酶，激酶，血液蛋白，趋化因子，细胞因子，抗体等。
-
3.发明申请Method for Rapidly Screening Microbial Hosts to Identify Certain Strains with Improved Yield and/or Quality in the Expression of Heterologous Proteins 有权快速筛选微生物宿主鉴定异源蛋白表达中产量和/或质量提高的某些菌株的方法公开(公告)号：US20100137162A1
公开(公告)日：2010-06-03
申请号：US12610207
申请日：2009-10-30
申请人： Diane M. Retallack , Charles H. Squires , Thomas M. Ramseier , Russell J. Coleman , Jane C. Schneider , Charies D. Hershberger
发明人： Diane M. Retallack , Charles H. Squires , Thomas M. Ramseier , Russell J. Coleman , Jane C. Schneider , Charies D. Hershberger
CPC分类号： C12N15/1037 , C12N15/78
摘要： The present invention provides an array for rapidly identifying a host cell population capable of producing heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment, or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest, including therapeutic proteins, hormones, a growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.
摘要翻译： 本发明提供了一种用于快速鉴定能够产生具有改善的产量和/或质量的异源蛋白质的宿主细胞群的阵列。 该阵列包含已被遗传修饰的一个或多个宿主细胞群，以增加涉及蛋白质产生的一个或多个靶基因的表达，减少参与蛋白质降解的一个或多个靶基因的表达，或两者。 阵列中的一个或多个菌株可以在周质隔室中表达感兴趣的异源蛋白质，或者通过外细胞壁在细胞外分泌异源蛋白质。 菌株阵列可用于筛选任何目的蛋白质的表达，包括治疗性蛋白质，激素，生长因子，细胞外受体或配体，蛋白酶，激酶，血液蛋白，趋化因子，细胞因子，抗体等。
-
4.发明授权Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins 有权用于快速筛选微生物宿主以鉴定具有提高异源蛋白质表达的产量和/或质量的某些菌株的方法公开(公告)号：US09580719B2
公开(公告)日：2017-02-28
申请号：US12610207
申请日：2009-10-30
申请人： Diane M. Retallack , Charles H. Squires , Thomas M. Ramseier , Russell J. Coleman , Jane C. Schneider , Charles D. Hershberger
发明人： Diane M. Retallack , Charles H. Squires , Thomas M. Ramseier , Russell J. Coleman , Jane C. Schneider , Charles D. Hershberger
IPC分类号： C12N15/78
CPC分类号： C12N15/1037 , C12N15/78
摘要： The present invention provides an array for rapidly identifying a host cell population capable of producing a heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest including therapeutic proteins, hormones, growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.
摘要翻译： 本发明提供了一种用于快速鉴定能够产生具有改善的产量和/或质量的异源蛋白质的宿主细胞群的阵列。 该阵列包含已被遗传修饰的一个或多个宿主细胞群，以增加参与蛋白质产生的一个或多个靶基因的表达，减少参与蛋白质降解的一个或多个靶基因的表达，或两者。 阵列中的一个或多个菌株可以在周质隔室中表达感兴趣的异源蛋白质，或者通过外细胞壁在细胞外分泌异源蛋白质。 菌株阵列可用于筛选任何目的蛋白质的表达，包括治疗性蛋白质，激素，生长因子，细胞外受体或配体，蛋白酶，激酶，血液蛋白，趋化因子，细胞因子，抗体等。
-
5.发明授权
公开(公告)号：US5811285A
公开(公告)日：1998-09-22
申请号：US715554
申请日：1996-09-19
IPC分类号： C12N15/09 , C10G31/00 , C10G32/00 , C12N1/20 , C12N1/21 , C12N9/02 , C12N9/04 , C12N15/52 , C12N15/53 , C12P1/00 , C12R1/01 , C12S1/02 , C12N15/00 , C07H21/04
CPC分类号： C12N1/20 , C10G31/00 , C10G32/00 , C12N15/52 , C12N9/0004 , C12N9/0006 , C12P1/00 , C12R1/01
摘要： The invention relates to the discovery that the rate of reaction of the desulfurization of fossil fuels is enhanced by the addition of an oxidoreductase to the biocatalyst. The invention is drawn to a method for enhancing the rate of desulfurizing a fossil fuel containing organic sulfur compounds, comprising the steps of: a) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of an oxidoreductase, thereby forming a fossil fuel and aqueous phase mixture; b) maintaining the mixture of step (a) under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and c) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase. The invention also relates to a recombinant microorganism containing one or more recombinant DNA molecules which encode a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and which encode an oxidoreductase. The invention also relates to a composition comprising (a) a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and (b) an oxidoreductase.
摘要翻译： 本发明涉及通过向生物催化剂中加入氧化还原酶来提高化石燃料脱硫反应速率的发现。 本发明涉及提高含有机硫化合物的化石燃料脱硫速率的方法，包括以下步骤：a）使化石燃料与含有能够裂解碳 - 硫键的生物催化剂的水相接触， 增加氧化还原酶的量，从而形成化石燃料和水相混合物; b）将步骤（a）的混合物保持在足以通过生物催化剂裂解有机硫分子的碳 - 硫键的条件下，从而导致具有降低的有机硫含量的化石燃料; 和c）从所得水相中分离具有降低的有机硫含量的化石燃料。 本发明还涉及含有一种或多种重组DNA分子的重组微生物，所述重组DNA分子编码能够脱硫含有有机硫分子的化石燃料并编码氧化还原酶的生物催化剂。 本发明还涉及一种组合物，其包含（a）能够使含有机硫分子的化石燃料脱硫的生物催化剂和（b）氧化还原酶。
-
公开(公告)号：US09708616B2
公开(公告)日：2017-07-18
申请号：US14974897
申请日：2015-12-18
摘要： Provided herein are methods and compositions for expression of a nucleic acid construct comprising nucleic acids encoding a) a recombinant polypeptide, and b) a prototrophy-restoring enzyme in a host cell that is auxotrophic for at least one metabolite. In various embodiments, the host cell is auxotrophic for a nitrogenous base compound or an amino acid. The invention involves introducing an analog into the growth media for the host cell such that the analog is incorporated into the recombinant polypeptide or a nucleic acid coding sequence thereof. In various embodiments, the compositions and methods disclosed herein result in improved recombinant protein expression compared to expression of recombinant protein in an antibiotic selection system, or compared to expression of the recombinant protein in an expression system that lacks a metabolite analog.
-
7.发明授权公开(公告)号：US09243253B2
公开(公告)日：2016-01-26
申请号：US13319844
申请日：2010-05-10
摘要： Provided herein are methods and compositions for expression of a nucleic acid construct comprising nucleic acids encoding a) a recombinant polypeptide, and b) a prototrophy-restoring enzyme in a host cell that is auxotrophic for at least one metabolite. In various embodiments, the host cell is auxotrophic for a nitrogenous base compound or an amino acid. The invention involves introducing an analogue into the growth media for the host cell such that the analogue is incorporated into the recombinant polypeptide or a nucleic acid coding sequence thereof. In various embodiments, the compositions and methods disclosed herein result in improved recombinant protein expression compared to expression of recombinant protein in an antibiotic selection system, or compared to expression of the recombinant protein in an expression system that lacks a metabolite analogue.
摘要翻译： 本文提供了用于表达包含编码a）重组多肽的核酸的核酸构建体的方法和组合物，以及b）对于至少一种代谢产物是营养缺陷型的宿主细胞中的原始营养恢复酶。 在各种实施方案中，宿主细胞对于含氮碱性化合物或氨基酸是营养缺陷型的。 本发明涉及将类似物引入宿主细胞的生长培养基中，使得类似物掺入重组多肽或其核酸编码序列中。 在各种实施方案中，与抗生素选择系统中的重组蛋白的表达相比，本文公开的组合物和方法导致改善的重组蛋白表达，或与缺乏代谢物类似物的表达系统中重组蛋白的表达进行比较。
-
公开(公告)号：US5919683A
公开(公告)日：1999-07-06
申请号：US105057
申请日：1998-06-26
CPC分类号： C12N9/0036 , C10G32/00
摘要： The invention provides a novel flavin reductase, which has been isolated from Rhodococcus strain sp. IGTS8 and purified to homogeneity. The flavin reductase is of use in the biocatalytic desulfurization of a fossil fuel containing organic sulfur compounds. The method comprises the steps of (1) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of the flavin reductase of the present invention, thereby forming a fossil fuel and aqueous phase mixture; (2) maintaining the mixture under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and (3) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase.
摘要翻译： 本发明提供了一种新的黄素还原酶，其已从红球菌属菌株sp。 IGTS8并纯化至均匀。 黄素还原酶在含有机硫化合物的化石燃料的生物催化脱硫中有用。 该方法包括以下步骤：（1）使化石燃料与含有能够切割碳 - 硫键的生物催化剂和速率增加量的本发明的黄素还原酶的水相接触，从而形成化石燃料和水相 混合物; （2）将混合物保持在足以通过生物催化剂裂解有机硫分子的碳 - 硫键的条件下，从而导致具有降低的有机硫含量的化石燃料; 和（3）从所得水相中分离具有降低的有机硫含量的化石燃料。
-
9.发明申请DESIGN FOR RAPIDLY CLONING ONE OR MORE POLYPEPTIDE CHAINS INTO AN EXPRESSION SYSTEM 审中-公开设计快速克隆一个或多个多肽链进入表达系统公开(公告)号：US20110020830A1
公开(公告)日：2011-01-27
申请号：US12933660
申请日：2009-03-31
CPC分类号： H04N5/225
摘要： The present invention provides methods and compositions for the generation and identification of expression constructs that can be used to express sufficient levels of a polypeptide of interest. The compositions include a population of expression vectors, wherein members of the population have a type IIS restriction enzyme recognition site adjacent to a regulatory sequence, and wherein the regulatory element is distinct in at least two members of the population of expression vectors. In various embodiments, the expression vectors further comprise a polynucleotide sequence encoding a polypeptide of interest, wherein the polynucleotide encoding the polypeptide, the polynucleotide of the regulatory sequence, or both, are distinct in at least two members of the population. The compositions are useful for identifying a combination of coding sequences and/or regulatory elements useful for the heterologous expression of the polypeptide of interest.
摘要翻译： 本发明提供了用于产生和鉴定可用于表达足够水平的感兴趣多肽的表达构建体的方法和组合物。 组合物包括一组表达载体，其中群体的成员具有与调节序列相邻的IIS型限制酶识别位点，并且其中调节元件在表达载体群体的至少两个成员中是不同的。 在各种实施方案中，表达载体还包含编码目的多肽的多核苷酸序列，其中编码多肽的多核苷酸，调节序列的多核苷酸或两者在群体的至少两个成员中是不同的。 该组合物可用于鉴定编码序列和/或对感兴趣的多肽的异源表达有用的调控元件的组合。
-
公开(公告)号：US5804433A
公开(公告)日：1998-09-08
申请号：US735963
申请日：1996-10-23
CPC分类号： C12N9/0036 , C10G32/00
摘要： The invention provides a novel flavin reductase, which has been isolated from Rhodococcus strain sp. IGTS8 and purified to homogeneity. The flavin reductase is of use in the biocatalytic desulfurization of a fossil fuel containing organic sulfur compounds. The method comprises the steps of (1) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of the flavin reductase of the present invention, thereby forming a fossil fuel and aqueous phase mixture; (2) maintaining the mixture under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and (3) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase.
摘要翻译： 本发明提供了一种新的黄素还原酶，其已从红球菌属菌株sp。 IGTS8并纯化至均匀。 黄素还原酶在含有机硫化合物的化石燃料的生物催化脱硫中有用。 该方法包括以下步骤：（1）使化石燃料与含有能够切割碳 - 硫键的生物催化剂和速率增加量的本发明的黄素还原酶的水相接触，从而形成化石燃料和水相 混合物; （2）将混合物保持在足以通过生物催化剂裂解有机硫分子的碳 - 硫键的条件下，从而导致具有降低的有机硫含量的化石燃料; 和（3）从所得水相中分离具有降低的有机硫含量的化石燃料。
-
-
-
-
-
-
-
-
-
搜索结果
20 条记录 (耗时 0.026 秒)