发明授权
- 专利标题： Methods for producing polypeptides in enzyme-deficient mutants of Fusarium venenatum
- 专利标题（中）： 在镰孢镰刀菌酶缺陷突变体中产生多肽的方法
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申请号： US13121254申请日： 2009-09-30
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公开(公告)号： US08647856B2公开(公告)日： 2014-02-11
- 发明人: Jeffrey Shasky , Wendy Yoder
- 申请人： Jeffrey Shasky , Wendy Yoder
- 申请人地址： US CA Davis
- 专利权人： Novozymes Inc.
- 当前专利权人： Novozymes Inc.
- 当前专利权人地址： US CA Davis
- 代理商 Elias Lambiris
- 国际申请： PCT/US2009/059039 WO 20090930
- 国际公布： WO2010/039840 WO 20100408
- 主分类号： C12N1/00
- IPC分类号： C12N1/00 ; C12N1/14
摘要：
The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.
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