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    Methods for producing polypeptides in enzyme-deficient mutants of Fusarium venenatum
    2.
    发明授权
    Methods for producing polypeptides in enzyme-deficient mutants of Fusarium venenatum 失效
    在镰孢镰刀菌酶缺陷突变体中产生多肽的方法

    公开(公告)号：US08647856B2

    公开(公告)日：2014-02-11

    申请号：US13121254

    申请日：2009-09-30

    申请人： Jeffrey Shasky ,  Wendy Yoder

    发明人： Jeffrey Shasky ,  Wendy Yoder

    IPC分类号： C12N1/00 ,  C12N1/14

    CPC分类号： C12P21/00 ,  C12N9/242 ,  C12N9/58 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02 ,  C12Y302/01001 ,  C12Y401/01023 ,  Y02P20/52

    摘要： The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.

    摘要翻译： 本发明涉及产生多肽的方法，其包括：（a）在用于产生多肽的培养基中培养亲本尖叶镰刀菌菌株的突变体，其中所述突变株包含编码多肽的多核苷酸和一个或多个（ 几种）选自pyrG，amyA和alpA的基因，其中所述一个或多个（若干）基因被修饰，使所述突变株缺乏产生选自下组的一种或多种（几种） -5'-单磷酸脱羧酶，α-淀粉酶和碱性蛋白酶，与相同条件下栽培的亲本镰刀菌镰孢菌株相比; 和（b）从培养基中回收多肽。 本发明还涉及镰刀菌菌株的酶缺陷型突变体及其生产方法。

    Methods for producing polypeptides in enzyme-deficient mutants of fusarium venenatum
    3.
    发明申请
    Methods for producing polypeptides in enzyme-deficient mutants of fusarium venenatum 失效
    在镰孢镰刀菌酶缺陷突变体中产生多肽的方法

    公开(公告)号：US20110236928A1

    公开(公告)日：2011-09-29

    申请号：US13121254

    申请日：2009-09-30

    申请人： Jeffrey Shasky ,  Wendy Yoder

    发明人： Jeffrey Shasky ,  Wendy Yoder

    IPC分类号： C12P21/00 ,  C12N1/15 ,  C12N15/80

    CPC分类号： C12P21/00 ,  C12N9/242 ,  C12N9/58 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02 ,  C12Y302/01001 ,  C12Y401/01023 ,  Y02P20/52

    摘要： The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.

    摘要翻译： 本发明涉及产生多肽的方法，其包括：（a）在用于产生多肽的培养基中培养亲本尖叶镰刀菌菌株的突变体，其中所述突变株包含编码多肽的多核苷酸和一个或多个（ 几种）选自pyrG，amyA和alpA的基因，其中所述一个或多个（若干）基因被修饰，使所述突变株缺乏产生选自下组的一种或多种（几种） -5'-单磷酸脱羧酶，α-淀粉酶和碱性蛋白酶，与相同条件下栽培的亲本镰刀菌镰孢菌株相比; 和（b）从培养基中回收多肽。 本发明还涉及镰刀菌菌株的酶缺陷型突变体及其生产方法。

    Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
    4.
    发明申请
    Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells 有权
    在单端孢霉烯缺乏丝状真菌突变体细胞中产生异源多肽的方法

    公开(公告)号：US20050181480A1

    公开(公告)日：2005-08-18

    申请号：US11095491

    申请日：2005-03-31

    申请人： John Royer ,  Lynne Christianson ,  Gregory Gambetta ,  Howard Brody ,  Suzanne Otani ,  Wendy Yoder

    发明人： John Royer ,  Lynne Christianson ,  Gregory Gambetta ,  Howard Brody ,  Suzanne Otani ,  Wendy Yoder

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N9/02 ,  C12N9/06 ,  C12N9/24 ,  C12N9/88 ,  C12N15/60 ,  C12N15/80 ,  C12P21/02 ,  C12R1/77 ,  C12P21/06 ,  C12N5/06 ,  C12N15/85

    CPC分类号： C12N9/0036 ,  C12N9/0044 ,  C12N9/2482 ,  C12N9/88 ,  C12N15/80 ,  C12Y302/01008 ,  C12Y402/03006

    摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less of the trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated trichodiene synthases and isolated nucleic acid sequences encoding the trichodiene synthases. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the trichodiene synthases. The present invention further relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells.

    摘要翻译： 本发明涉及生产多肽的方法，其包括：（a）在有利于产生多肽的条件下培养亲本丝状真菌细胞的突变体，其中（i）突变细胞包含编码多肽的第一核酸序列 多肽和第二核酸序列，其包含参与单端孢霉烯生产的至少一个基因的修饰;和（ii）当在相同条件下培养时，所述突变体产生比亲本丝状真菌细胞少的单端孢霉烯; 和（b）从培养基中分离多肽。 本发明还涉及丝状真菌细胞的突变体和获得突变细胞的方法。 本发明还涉及分离的二恶烯合成酶和分离的编码二萜烯合酶的核酸序列。 本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及产生二氢合酶合酶的方法。 本发明还涉及包含基因的无标记物修饰的突变体细胞，以及获得和使用这种突变细胞的方法。