- 专利标题: Methods and compositions for multiplex PCR
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申请号: US13618805申请日: 2012-09-14
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公开(公告)号: US08673560B2公开(公告)日: 2014-03-18
- 发明人: John Leamon , Mark Andersen , Michael Thornton
- 申请人: John Leamon , Mark Andersen , Michael Thornton
- 申请人地址: US CA Carlsbad
- 专利权人: Life Technologies Corporation
- 当前专利权人: Life Technologies Corporation
- 当前专利权人地址: US CA Carlsbad
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12P19/34 ; C07H21/02 ; C07H21/04
摘要:
The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.
公开/授权文献
- US20130011881A1 METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 公开/授权日:2013-01-10