公开(公告)号：US08978939B2

公开(公告)日：2015-03-17

申请号：US11573715

申请日：2005-08-09

申请人： Martin C. Bull ,  John Leamon

发明人： Martin C. Bull ,  John Leamon

IPC分类号： B65D5/72 ,  B65D47/20

CPC分类号： B65D47/2031 ,  B65D2547/066

摘要： A retaining device (1) has a non-laminar self-closing valve (2) retained therein. The valve comprises a flange (8), and the rigidity of the flange (8) is increased by the device (1). The retaining device is a single-piece article.

摘要翻译： 保持装置（1）具有保持在其中的非层流自闭阀（2）。 该阀包括凸缘（8），凸缘（8）的刚度由装置（1）增加。 保持装置是单件制品。

公开(公告)号：US08728736B2

公开(公告)日：2014-05-20

申请号：US13619178

申请日：2012-09-14

申请人： John Leamon ,  Mark Andersen ,  Michael Thornton

发明人： John Leamon ,  Mark Andersen ,  Michael Thornton

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/686 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

摘要： The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

摘要翻译： 本发明提供可用于样品中存在的一种或多种核酸的多重PCR的方法，组合物，试剂盒，系统和装置。 特别地，提供允许一个或多个靶序列的选择性扩增的各种靶特异性引物。 一方面，本发明涉及可用于选择性扩增与癌症或遗传性疾病相关的一种或多种靶序列的靶特异性引物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的扩增靶序列可用于包括核酸测序在内的各种下游过程，并用于检测遗传变体的存在。

公开(公告)号：US08673560B2

公开(公告)日：2014-03-18

申请号：US13618805

申请日：2012-09-14

申请人： John Leamon ,  Mark Andersen ,  Michael Thornton

发明人： John Leamon ,  Mark Andersen ,  Michael Thornton

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/686 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

摘要： The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

公开(公告)号：US08470164B2

公开(公告)日：2013-06-25

申请号：US13001182

申请日：2009-06-25

申请人： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo ,  John Leamon ,  Jonathan Schultz

发明人： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo ,  John Leamon ,  Jonathan Schultz

IPC分类号： G01N27/414 ,  C12Q1/68

CPC分类号： C12Q1/6874 ,  B01L3/502761 ,  B01L2300/046 ,  B01L2300/0663 ,  B01L2300/0851 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N27/4148 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2565/629

摘要： Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

公开(公告)号：US20110201523A1

公开(公告)日：2011-08-18

申请号：US13027336

申请日：2011-02-15

申请人： Wolfgang HINZ ,  John Leamon ,  David Light ,  Jonathan Rothberg

发明人： Wolfgang HINZ ,  John Leamon ,  David Light ,  Jonathan Rothberg

IPC分类号： C40B40/06

CPC分类号： C12N15/1093 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

摘要： The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

摘要翻译： 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中颗粒对颗粒的变化减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。

公开(公告)号：US20110201508A1

公开(公告)日：2011-08-18

申请号：US13029566

申请日：2011-02-17

申请人： WOLFGANG HINZ ,  JOHN LEAMON ,  DAVID LIGHT ,  JONATHAN ROTHBERG

发明人： WOLFGANG HINZ ,  JOHN LEAMON ,  DAVID LIGHT ,  JONATHAN ROTHBERG

IPC分类号： C40B30/00 ,  C12Q1/68

CPC分类号： C12N15/1093 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

摘要： The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

摘要翻译： 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中粒子与粒子变化的减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。

公开(公告)号：US20110195252A1

公开(公告)日：2011-08-11

申请号：US13026707

申请日：2011-02-14

申请人： Wolfgang HINZ ,  John Leamon ,  David Light ,  Jonathan Rothberg

发明人： Wolfgang HINZ ,  John Leamon ,  David Light ,  Jonathan Rothberg

IPC分类号： B32B5/00 ,  C08F265/10

CPC分类号： C12N15/1093 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

摘要： The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

摘要翻译： 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中粒子与粒子变化的减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。

公开(公告)号：US09957558B2

公开(公告)日：2018-05-01

申请号：US13458739

申请日：2012-04-27

申请人： John Leamon ,  Mark Andersen ,  Michael Thornton

发明人： John Leamon ,  Mark Andersen ,  Michael Thornton

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/686 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

摘要： The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

公开(公告)号：US08574835B2

公开(公告)日：2013-11-05

申请号：US12785685

申请日：2010-05-24

申请人： Wolfgang Hinz ,  John Leamon ,  David Light ,  Jonathan Rothberg

发明人： Wolfgang Hinz ,  John Leamon ,  David Light ,  Jonathan Rothberg

IPC分类号： C12Q1/68 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00

CPC分类号： B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12N15/1093 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

摘要： The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

摘要翻译： 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中粒子与粒子变化的减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。

公开(公告)号：US20110217697A1

公开(公告)日：2011-09-08

申请号：US13001182

申请日：2009-06-25

申请人： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo ,  John Leamon ,  Jonathan Schultz

发明人： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo ,  John Leamon ,  Jonathan Schultz

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  B01L3/502761 ,  B01L2300/046 ,  B01L2300/0663 ,  B01L2300/0851 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N27/4148 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2565/629

摘要： Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

摘要翻译： 与用于分析物测量的非常大规模的FET阵列相关的方法和装置。 可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET（例如，ISFET）阵列，并且同时促进显着小的像素尺寸和致密阵列。 改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。 在一个示例中，chemFET阵列基于监测无机焦磷酸盐（PPi），氢离子和核苷酸三磷酸盐的浓度变化来促进DNA测序技术。