发明授权
- 专利标题： Archaeon expression system
- 专利标题（中）： 古生物表达系统
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申请号： US10559583申请日： 2004-06-02
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公开(公告)号： US08679829B2公开(公告)日： 2014-03-25
- 发明人: Christa Schleper , Melanie Jonuscheit , Jürgen Eck , Frank Niehaus , Sonja-Verena Albers , Sabrina Froels
- 申请人： Christa Schleper , Melanie Jonuscheit , Jürgen Eck , Frank Niehaus , Sonja-Verena Albers , Sabrina Froels
- 申请人地址： DE
- 专利权人： B.R.A.I.N. Biotechnology Research and Information Network AG
- 当前专利权人： B.R.A.I.N. Biotechnology Research and Information Network AG
- 当前专利权人地址： DE
- 代理机构： Pillsbury Winthrop Shaw Pittman LLP
- 优先权： EP03012552 20030602
- 国际申请： PCT/EP2004/005936 WO 20040602
- 国际公布： WO2004/106527 WO 20041209
- 主分类号： C12N15/00
- IPC分类号： C12N15/00 ; C12N15/866 ; C12N15/70 ; C12N15/11
摘要：
The present invention relates to a sulfolobus expression vector comprising: (a) sulfolobus origin of replication; (b) the genes encoding the structural proteins and the site-specific integrase of SSV1, SSV2 or pSSVx, operatively linked to expression control sequences and a packaging signal; (c) one or more selectable marker gene(s), operatively linked to sulfolobus expression control sequences; and (d) a sulfolobus promoter followed 3′ by a restriction enzyme recognition site or a multiple cloning site for insertion of a gene of interest and optionally a 3′ regulatory element. Moreover, the present invention relates to a shuttle vector comprising the sequences of the expression vector of the invention and additional sequences for propagation and selection in E. coli, wherein the additional sequences comprise (a) an E. coli on of replication; and (b) a marker for selection in E. coli. Furthermore, the invention relates to host cells transformed with the expression vector as well as to a kit comprising a vector or a host cell of the present invention. Finally, the present application also relates to a method for generating infectious subviral particles.
公开/授权文献
- US20070134201A1 Archaeon expression system 公开/授权日：2007-06-14
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