Invention Grant
- Patent Title: Methods of increasing secretion of polypeptides having biological activity
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Application No.: US14024292Application Date: 2013-09-11
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Publication No.: US08735549B2Publication Date: 2014-05-27
- Inventor: Sandra Merino
- Applicant: Novozymes, Inc.
- Applicant Address: US CA Davis
- Assignee: Novozymes, Inc.
- Current Assignee: Novozymes, Inc.
- Current Assignee Address: US CA Davis
- Agent Robert L. Starnes
- Main IPC: C12P21/00
- IPC: C12P21/00 ; C12P21/04 ; C07K1/00 ; C12P21/02

Abstract:
The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.
Public/Granted literature
- US20140011256A1 Methods of increasing secretion of polypeptides having biological activity Public/Granted day:2014-01-09
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