An exemplary locomotion analysis method includes steps of: acquiring a depth map including an image of a measured object, filtering out a background image of the depth map according to a depth threshold, finding out the image of the measured object from the residual image of the depth map, calculating three-dimensional (3D) coordinates of the measured object according to the image of the measured object has been found out, recording the 3D coordinates to reconstruct a 3D moving track of the measured object and performing a locomotion analysis of the measured object according to the 3D moving track. Moreover, an exemplary locomotion analysis apparatus applied to the above method also is provided.