A method for 3D video content generation is disclosed, capable of transforming a 2D image into a 3D video through proper operation process. The method comprises the following steps of: (A) receiving a 2D image and generating a ROI distribution map from the 2D image; (B) executing a color feature capture process, for forming a plural of color feature regions; (C) executing an image segmentation process basing on the texture feature of the plural of color feature regions, for forming an image region distribution map; (D) executing a depth map generation process, for generating a depth map basing on the ROI distribution map and the image region distribution map; (E) executing a 3D image generation process, for forming the 3D image basing on the image region distribution map and the depth map; and (F) chaining a plurality of the 3D images to form the 3D video basing on a frame rate.