发明授权
- 专利标题: Methods for quantitative cDNA analysis in single-cell
- 专利标题(中): 单细胞定量cDNA分析方法
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申请号: US11783575申请日: 2007-04-10
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公开(公告)号: US08802367B2公开(公告)日: 2014-08-12
- 发明人: Kiyomi Taniguchi , Hideki Kambara , Tomoharu Kajiyama
- 申请人: Kiyomi Taniguchi , Hideki Kambara , Tomoharu Kajiyama
- 申请人地址: JP Tokyo
- 专利权人: Hitachi, Ltd.
- 当前专利权人: Hitachi, Ltd.
- 当前专利权人地址: JP Tokyo
- 代理机构: Stites & Harbison, PLLC.
- 代理商 Stephen J. Weyer, Esq.
- 优先权: JP2006-150189 20060530
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12P19/34 ; C07H21/02 ; C07H21/04
摘要:
It is an object to provide a method of suitably analyzing the amount of gene expression of a single-cell.A method of detecting a nucleic acid comprising a step of sampling a single-cell from a sample containing at least a single-cell, a cell lysis step of lysing cell membrane of the sampled single-cell and extracting nucleic acids from the cell, a DNase treatment step of degrading DNA of the extracted nucleic acids with DNase, a step of hybridizing mRNA of the total RNA contained in the single-cell with oligo (dT) fixed onto a carrier, a step of performing reverse transcription of the mRNA hybridized with the oligo (dT) to fix cDNA derived from the single-cell onto the carrier, thereby preparing a single-cell derived cDNA library fixed onto a carrier, and a step of amplifying cDNA fixed onto the carrier and simultaneously detecting an amplification amount of the cDNA.
公开/授权文献
- US20070281313A1 Methods for quantitative cDNA analysis in single-cell 公开/授权日:2007-12-06