-
公开(公告)号：US20070281313A1
公开(公告)日：2007-12-06
申请号：US11783575
申请日：2007-04-10
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6811 , C12N15/1096 , C12Q1/6809 , C12Q1/6834 , C12Q2565/537 , C12Q2525/173 , C12Q2521/301 , C12Q2561/113
摘要： It is an object to provide a method of suitably analyzing the amount of gene expression of a single-cell.A method of detecting a nucleic acid comprisinga step of sampling a single-cell from a sample containing at least a single-cell,a cell lysis step of lysing cell membrane of the sampled single-cell and extracting nucleic acids from the cell,a DNase treatment step of degrading DNA of the extracted nucleic acids with DNase,a step of hybridizing mRNA of the total RNA contained in the single-cell with oligo (dT) fixed onto a carrier,a step of performing reverse transcription of the mRNA hybridized with the oligo (dT) to fix cDNA derived from the single-cell onto the carrier, thereby preparing a single-cell derived cDNA library fixed onto a carrier, anda step of amplifying cDNA fixed onto the carrier and simultaneously detecting an amplification amount of the cDNA.
摘要翻译： 本发明的目的是提供适当分析单细胞的基因表达量的方法。 一种检测核酸的方法，包括从含有至少单细胞的样品中取样单细胞的步骤，裂解采样的单细胞的细胞膜并从细胞中提取核酸的细胞裂解步骤 用DNase降解提取的核酸的DNA的DNA酶处理步骤，将单细胞中含有的总RNA的mRNA与固定在载体上的寡聚（dT）杂交的步骤，将与mRNA杂交的mRNA进行逆转录的步骤 将来自单细胞的cDNA固定在载体上的寡核苷酸（dT），从而制备固定在载体上的单细胞衍生的cDNA文库，以及扩增固定在载体上的cDNA并同时检测扩增量的步骤 cDNA。
-
公开(公告)号：US11053536B2
公开(公告)日：2021-07-06
申请号：US14418135
申请日：2012-07-30
IPC分类号： C12Q1/6837 , C12N15/10
摘要： The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.
-
3.发明申请Tag-Sequence-Attached Two-Dimensional CDNA Library Device, and Gene Expression Analysis Method and Gene Expression Analysis Apparatus Each Utilizing Same 审中-公开标签序列连接的二维CDNA文库装置及基因表达分析方法及基因表达分析装置公开(公告)号：US20150167063A1
公开(公告)日：2015-06-18
申请号：US14418135
申请日：2012-07-30
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12N15/1096 , C12Q2525/155 , C12Q2565/537 , C12Q2565/601
摘要： The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.
摘要翻译： 本发明涉及用于分析单细胞中基因表达的方法，装置和装置。 具体地说，本发明涉及：用于基因表达分析的装置，其特征在于，包括载体，其中具有测试核酸捕获序列和已知序列的核酸探针，并且还包含不同的细胞识别标签序列 关于载体表面或其表面附近的位置差异，具有已知序列的常见引物序列被二维分布和固定在载体的表面上或其表面附近 ; 以及使用该基因表达分析装置的方法和装置。
-
公开(公告)号：US08802367B2
公开(公告)日：2014-08-12
申请号：US11783575
申请日：2007-04-10
CPC分类号： C12Q1/6811 , C12N15/1096 , C12Q1/6809 , C12Q1/6834 , C12Q2565/537 , C12Q2525/173 , C12Q2521/301 , C12Q2561/113
摘要： It is an object to provide a method of suitably analyzing the amount of gene expression of a single-cell.A method of detecting a nucleic acid comprising a step of sampling a single-cell from a sample containing at least a single-cell, a cell lysis step of lysing cell membrane of the sampled single-cell and extracting nucleic acids from the cell, a DNase treatment step of degrading DNA of the extracted nucleic acids with DNase, a step of hybridizing mRNA of the total RNA contained in the single-cell with oligo (dT) fixed onto a carrier, a step of performing reverse transcription of the mRNA hybridized with the oligo (dT) to fix cDNA derived from the single-cell onto the carrier, thereby preparing a single-cell derived cDNA library fixed onto a carrier, and a step of amplifying cDNA fixed onto the carrier and simultaneously detecting an amplification amount of the cDNA.
摘要翻译： 本发明的目的是提供适当分析单细胞的基因表达量的方法。 一种检测核酸的方法，包括从含有至少单细胞的样品中取样单细胞的步骤，裂解采样的单细胞的细胞膜并从细胞中提取核酸的细胞裂解步骤 用DNase降解提取的核酸的DNA的DNA酶处理步骤，将单细胞中含有的总RNA的mRNA与固定在载体上的寡聚（dT）杂交的步骤，将与mRNA杂交的mRNA进行逆转录的步骤 将来自单细胞的cDNA固定在载体上的寡核苷酸（dT），从而制备固定在载体上的单细胞衍生的cDNA文库，以及扩增固定在载体上的cDNA并同时检测扩增量的步骤 cDNA。
-
公开(公告)号：US06734557B2
公开(公告)日：2004-05-11
申请号：US10369760
申请日：2003-02-21
IPC分类号： H01L2348
CPC分类号： H05K3/3452 , H01L21/4853 , H01L23/49838 , H01L24/48 , H01L2224/0401 , H01L2224/05599 , H01L2224/32225 , H01L2224/48091 , H01L2224/48227 , H01L2224/48464 , H01L2224/73265 , H01L2224/85399 , H01L2924/00014 , H01L2924/01004 , H01L2924/01005 , H01L2924/01006 , H01L2924/01028 , H01L2924/01029 , H01L2924/01078 , H01L2924/01079 , H01L2924/014 , H01L2924/15311 , H01L2924/181 , H05K1/113 , H05K2201/09527 , H05K2201/0989 , H01L2924/00 , H01L2224/45099 , H01L2924/00012 , H01L2224/45015 , H01L2924/207
摘要： A semiconductor device comprising: a substrate; first and second interconnection patterns respectively provided on upper and lower surfaces of the substrate; a through-hole electrode extending through the substrate for electrically connecting the first and second interconnection patterns; a semiconductor chip provided on the upper surface of the substrate and electrically connected to the first interconnection pattern; and a resist film covering the second interconnection pattern; the second interconnection pattern comprising a generally round land and a lead interconnection portion extending from the land, the resist film having an opening formed therein for exposing the entire land, the opening having a curved edge surrounding a peripheral edge of the land and a linear edge linearly extending along a boundary between the land and the lead interconnection portion, the exposed land having a solder ball as an external terminal thereon.
摘要翻译： 一种半导体器件，包括：衬底; 分别设置在基板的上表面和下表面上的第一和第二互连图案; 延伸穿过所述基板的用于电连接所述第一和第二互连图案的通孔电极; 设置在所述基板的上表面并电连接到所述第一互连图案的半导体芯片; 以及覆盖所述第二互连图案的抗蚀剂膜; 所述第二互连图案包括大致圆形的平台和从所述焊盘延伸的引线互连部分，所述抗蚀剂膜具有形成在其中的开口，用于暴露所述整个焊盘，所述开口具有围绕所述焊盘边缘的弯曲边缘， 沿着焊盘和引线互连部分之间的边界线性延伸，暴露的焊盘在其上具有焊球作为外部端子。
-
公开(公告)号：US5196215A
公开(公告)日：1993-03-23
申请号：US814192
申请日：1991-12-20
IPC分类号： A21D13/00
CPC分类号： A21D13/0041
摘要： A composition for use in cavity foods as breads, cakes and other cavity foods suitable for filling to prevent softening of bread-dough by forming uniform cavities and form films preventing the transfer of water and oil thereto on filling is disclosed. The composition comprises (by weight) 10% to 30% of edible oils and fats, 2% to 25% of polysaccharides, 0.1% to 5% of edible emulsifiers, and 40% to 88% of water.
摘要翻译： 公开了一种用于空心食品的组合物，其用作面包，蛋糕和其它适合于通过形成均匀空腔而填充以防止面包生面团软化的空腔食品，并且在填充时形成防止水和油转移的膜。 该组合物包含（重量）10％至30％的食用油和脂肪，2％至25％的多糖，0.1％至5％的可食用乳化剂和40％至88％的水。
-
公开(公告)号：US5192568A
公开(公告)日：1993-03-09
申请号：US811717
申请日：1991-12-20
摘要： A composition for use in cavity foods as breads, cakes and other cavity foods suitable for filling to prevent softening of their dough by forming uniform cavities and forming films preventing the transfer of water and oil thereto on filling is disclosed. The composition comprises (by weight) 10% to 30% of edible oils and fats, 1% to 15%, preferably 1% to 10% proteins, 1% to 10% of polysaccharides, 0.1% to 5% phosphates, citrates or mixtures thereof, and 40% to 88% water.
摘要翻译： 公开了一种用于空腔食品的面包，蛋糕和其他空腔食品的组合物，其适用于通过形成均匀的空腔来填充以防止其面团的软化，并且在填充时形成防止水和油转移的薄膜。 组合物包含（重量）10％至30％的食用油和脂肪，1％至15％，优选1％至10％的蛋白质，1％至10％的多糖，0.1％至5％的磷酸盐，柠檬酸盐或混合物 和40％至88％的水。
-
公开(公告)号：US08389246B2
公开(公告)日：2013-03-05
申请号：US12292800
申请日：2008-11-26
申请人： Kiyomi Taniguchi , Hideki Kambara
发明人： Kiyomi Taniguchi , Hideki Kambara
IPC分类号： C12P19/34
CPC分类号： C12Q1/6851 , C12Q2545/101 , C12Q2525/161
摘要： It is intended to provide a novel convenient approach for DNA quantitative analysis that overcomes the disadvantages of conventional formulations. A standard DNA sample is prepared by introducing a single-base substitution into target DNA, and a predetermined amount thereof is mixed with a target DNA sample. The target and standard DNAs are amplified using the same primers designed to amplify a region comprising the single-base substitution site. To a hybridization product of a probe capable of binding to a site immediately before the single-base substitution site, ddATP, ddGTP, ddCTP, and ddTTP are sequentially added one by one to perform a complementary strand synthesis reaction. Luciferase reaction-induced luminescence derived from the formed pyrophosphoric acid is detected. The target DNA is quantitated from the amount of the detected luminescence and the amount of the added standard DNA sample.
-
公开(公告)号：US20090142767A1
公开(公告)日：2009-06-04
申请号：US12292800
申请日：2008-11-26
申请人： Kiyomi Taniguchi , Hideki Kambara
发明人： Kiyomi Taniguchi , Hideki Kambara
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2545/101 , C12Q2525/161
摘要： It is intended to provide a novel convenient approach for DNA quantitative analysis that overcomes the disadvantages of conventional formulations. A standard DNA sample is prepared by introducing a single-base substitution into target DNA, and a predetermined amount thereof is mixed with a target DNA sample. The target and standard DNAs are amplified using the same primers designed to amplify a region comprising the single-base substitution site. To a hybridization product of a probe capable of binding to a site immediately before the single-base substitution site, ddATP, ddGTP, ddCTP, and ddTTP are sequentially added one by one to perform a complementary strand synthesis reaction. Luciferase reaction-induced luminescence derived from the formed pyrophosphoric acid is detected. The target DNA is quantitated from the amount of the detected luminescence and the amount of the added standard DNA sample.
摘要翻译： 旨在为DNA定量分析提供一种新颖的方便方法，克服了常规制剂的缺点。 通过将单碱基置换引入目标DNA中制备标准DNA样品，并将其预定量与目标DNA样品混合。 使用设计用于扩增包含单碱基取代位点的区域的相同引物扩增靶标和标准DNA。 对于能够在单碱基置换位点之前结合位点的探针的杂交产物，逐个依次加入ddATP，ddGTP，ddCTP和ddTTP以进行互补链合成反应。 检测到由形成的焦磷酸衍生的荧光素酶反应诱导的发光。 从所检测的发光量和添加的标准DNA样品的量定量靶DNA。
-
10.发明申请公开(公告)号：US20120245053A1
公开(公告)日：2012-09-27
申请号：US13513605
申请日：2010-11-29
IPC分类号： C40B30/04
CPC分类号： C12N15/1093 , C12Q1/6809 , C12Q1/6837 , C40B40/08 , C40B50/06 , G01N33/54306 , G01N2458/10 , C12Q2531/125 , C12Q2533/107 , C12Q2565/537
摘要： The present invention provides a method and/or means for collecting and analyzing an individual cell in a tissue, and at the same time, quantitatively monitoring the expression levels of various genes while keeping two-dimensional information in the tissue. Specifically, the present invention provides a method comprising preparing a cDNA library from mRNA while keeping two-dimensional cellular distribution information and obtaining the gene expression levels at any site or all sites at a level of single cell. More specifically, the present invention provides a method comprising preparing a cDNA library in a sheet-form from mRNA while keeping two-dimensional cellular distribution information and repeatedly using the cDNA library in the detection of the gene expression, thereby allowing measurement of the expression distribution for a number of genes at a high accuracy.
摘要翻译： 本发明提供了一种用于收集和分析组织中的单个细胞的方法和/或方法，并且同时定量监测各种基因的表达水平，同时保持组织中的二维信息。 具体地说，本发明提供一种方法，其包括从mRNA制备cDNA文库，同时保持二维细胞分布信息，并在单细胞水平的任何位点或所有位点获得基因表达水平。 更具体地，本发明提供了一种方法，其包括从mRNA制备片段的cDNA文库，同时保持二维细胞分布信息，并在检测基因表达中重复使用cDNA文库，从而允许测量表达分布 对于许多基因在高精度。
-
-
-
-
-
-
-
-
-
搜索结果
12 条记录 (耗时 0.014 秒)
