发明授权
US08802929B2 MIR164 gene that controls plant root system development and fertility and use thereof
有权
MIR164基因,其控制植物根系发育和生育力及其使用
- 专利标题: MIR164 gene that controls plant root system development and fertility and use thereof
- 专利标题(中): MIR164基因,其控制植物根系发育和生育力及其使用
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申请号: US13319534申请日: 2010-05-12
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公开(公告)号: US08802929B2公开(公告)日: 2014-08-12
- 发明人: Lizhong Xiong , Kabin Xie , Xin Hou
- 申请人: Lizhong Xiong , Kabin Xie , Xin Hou
- 申请人地址: CN Hubei Province
- 专利权人: Huazhong Agricultural University
- 当前专利权人: Huazhong Agricultural University
- 当前专利权人地址: CN Hubei Province
- 代理机构: Thompson Coburn LLP
- 代理商 J. Wendy Davis
- 优先权: CN200910062045 20090513
- 国际申请: PCT/CN2010/000677 WO 20100512
- 国际公布: WO2010/130155 WO 20101118
- 主分类号: A01H5/00
- IPC分类号: A01H5/00 ; C12N15/82 ; C12N15/113
摘要:
A rice microRNA, miR164 gene, that controls plant root system development and fertility, is obtained through gene isolation, cloning and function verification. Uses of a nucleic acid fragment comprising miR164, which fragment may confer a transformed plant with the ability to increase root number and to alter fertility, wherein the said nucleic acid fragment is selected from one of the following nucleotide sequences: 1) a DNA sequence as shown in SEQ ID NO: 1; 2) a RNA sequence as shown in SEQ ID NO:2; or 3) the conserved sequence of miR164 having the same function as 1) or 2). The nucleotide sequence containing the precursor of miR164 is ligated with an exogenous promoter and introduced into rice to obtain transgenic rice plants which has large root systems but became infertile. The fertility can be restored by external application of phytohormones.