发明授权
US08809518B2 Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers
有权
包含可变内聚部分的寡核苷酸接头和通过使用所述接头制备多核苷酸文库的方法
- 专利标题： Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers
- 专利标题（中）： 包含可变内聚部分的寡核苷酸接头和通过使用所述接头制备多核苷酸文库的方法
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申请号： US12897745申请日： 2010-10-04
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公开(公告)号： US08809518B2公开(公告)日： 2014-08-19
- 发明人: Yoshihide Hayashizaki
- 申请人： Yoshihide Hayashizaki
- 申请人地址： JP Saitama
- 专利权人： Riken
- 当前专利权人： Riken
- 当前专利权人地址： JP Saitama
- 代理机构： Birch, Stewart, Kolasch & Birch, LLP
- 优先权： JP2000-306749 20001005
- 主分类号： C07H21/00
- IPC分类号： C07H21/00 ; C12Q1/68 ; C12P19/34
摘要：
The present invention relates to a linker or population of linkers that include an oligonucleotide fixed portion and an oligonucleotide variable portion represented by formula (N)n, wherein N is A, C, G, T or U, or their derivatives, and n is an integer equal to or higher than 1. A linker-polynucleotide or a population of linker-polynucleotides of the invention may be constituted by said linker or population of linkers and a target first strand polynucleotide bound to said linker. The invention also encompasses a method of preparing said linker or population of linkers and a method of preparing a linker-polynucleotide using said linker or population of linkers. The linkers or polynucleotide-linkers of the invention can be used in a method of preparing a cDNA library.
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