The present invention provides a transmission device and transmission method for glass substrate. The transmission device includes a transmission channel and a calibration mechanism; wherein the transmission channel for loading and transmitting, the calibration mechanism being disposed next to the transmission channel and the calibration mechanism comprising: a first positioning pillar, a second positioning pillar and a driver module; the first positioning pillar being disposed on one side of the transmission channel and the second positioning pillar being disposed on the other side of the transmission channel; the driver module driving the first positioning pillar and the second positioning pillar through the conveyor belt to move towards or away from each other so as to calibrate the position of the glass substrate by clamping or releasing the glass substrate during transmission. The present invention can solve the position deviation problem during transmitting glass substrate.