Invention Grant
US08835110B2 DNA integrity assay (DIA) for cancer diagnostics, using confocal fluorescence spectroscopy
有权
DNA完整性检测（DIA）用于癌症诊断，使用共焦荧光光谱法
- Patent Title: DNA integrity assay (DIA) for cancer diagnostics, using confocal fluorescence spectroscopy
- Patent Title (中): DNA完整性检测（DIA）用于癌症诊断，使用共焦荧光光谱法
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Application No.: US13120060Application Date: 2009-11-04
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Publication No.: US08835110B2Publication Date: 2014-09-16
- Inventor: Tza-Huei Wang , Kelvin J. Liu , Ie-Ming Shih
- Applicant: Tza-Huei Wang , Kelvin J. Liu , Ie-Ming Shih
- Applicant Address: US MD Baltimore
- Assignee: The Johns Hopkins University
- Current Assignee: The Johns Hopkins University
- Current Assignee Address: US MD Baltimore
- Agency: Venable LLP
- Agent Nancy J. Axelrod; Henry J. Daley
- International Application: PCT/US2009/063262 WO 20091104
- International Announcement: WO2010/053980 WO 20100514
- Main IPC: C12Q1/68
- IPC: C12Q1/68
Abstract:
The present invention relates, e.g., to a method for determining the size distribution of DNA molecules in a sample comprising cell-free nucleic acid, comprising labeling the DNA with a fluorescent dye in a stoichiometric manner, subjecting the DNA to molecular spectroscopy (e.g., cylindrical illumination confocal spectroscopy), analyzing suitable fluorescent burst parameters of the labeled DNA, and conducting single molecule DNA integrity analysis of the labeled DNA molecules in the sample. In one embodiment of the invention, the method is used as a diagnostic method for detecting cancer.
Public/Granted literature
- US20110171741A1 DNA INTEGRITY ASSAY (DIA) FOR CANCER DIAGNOSTICS, USING CONFOCAL FLUORESCENCE SPECTROSCOPY Public/Granted day:2011-07-14
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