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    Systems and methods for high-throughput microfluidic bead production
    1.
    发明授权
    Systems and methods for high-throughput microfluidic bead production 有权
    用于高通量微流珠生产的系统和方法

    公开(公告)号：US09156189B2

    公开(公告)日：2015-10-13

    申请号：US13822983

    申请日：2011-10-03

    申请人： Tza-Huei Wang ,  Weijie Beh ,  Dara L. Kraitchman ,  Hsa-Quan Mao

    发明人： Tza-Huei Wang ,  Weijie Beh ,  Dara L. Kraitchman ,  Hsa-Quan Mao

    IPC分类号： A61B5/055 ,  B29B9/12 ,  B01J13/00 ,  A61K9/16

    CPC分类号： B29B9/12 ,  A61K9/16 ,  B01J13/0052

    摘要： A system for producing microbeads includes a microfluidic device defining a supply channel and a shearing channel, a microbead precursor material disposed in the supply channel, a carrier fluid disposed in the shearing channel, and a pressure distribution system fluidly connected to each of the supply channel and the shearing channel to control at least relative pressures of the microbead precursor material and the carrier fluid. The supply channel includes a check valve adapted to be subjected to a bias pressure that is sufficient to close the check valve to flow of microbead precursor material when a supply pressure of the microbead precursor material is below a threshold pressure and is open to flow of the microbead precursor material when the supply pressure of the microbead precursor material is greater than the threshold pressure. An end of the supply channel opens into the shearing channel such that the microbead precursor material is sheared into droplets by the carrier fluid flowing through the shearing channel. A pressure of the carrier fluid is less than the bias pressure. The microbead precursor material and the carrier fluid are substantially immiscible.

    摘要翻译： 用于生产微珠的系统包括限定供应通道和剪切通道的微流体装置，设置在供应通道中的微珠前体材料，设置在剪切通道中的载体流体和流体连接到每个供应通道的压力分配系统 和剪切通道以控制微珠前体材料和载体流体的至少相对压力。 供应通道包括止回阀，该止回阀适于当微珠前体材料的供应压力低于阈值压力时足以关闭止回阀以流过微珠前体材料的偏压，并且对于 当微珠前体材料的供应压力大于阈值压力时，微珠前体材料。 供应通道的一端通向剪切通道，使得微珠前体材料被流过剪切通道的载体流体剪切成液滴。 载液的压力小于偏压。 微珠前体材料和载体流体基本上是不混溶的。

    SYSTEMS AND METHODS FOR HIGH-THROUGHPUT MICROFLUIDIC BEAD PRODUCTION
    2.
    发明申请
    SYSTEMS AND METHODS FOR HIGH-THROUGHPUT MICROFLUIDIC BEAD PRODUCTION 有权
    用于高通量微流控珠粒生产的系统和方法

    公开(公告)号：US20130183246A1

    公开(公告)日：2013-07-18

    申请号：US13822983

    申请日：2011-10-03

    申请人： Tza-Huei Wang ,  Weijie Beh ,  Dara L. Kraitchman ,  Hsa-Quan Mao

    发明人： Tza-Huei Wang ,  Weijie Beh ,  Dara L. Kraitchman ,  Hsa-Quan Mao

    IPC分类号： B29B9/12

    CPC分类号： B29B9/12 ,  A61K9/16 ,  B01J13/0052

    摘要： A system for producing microbeads includes a microfluidic device defining a supply channel and a shearing channel, a microbead precursor material disposed in the supply channel, a carrier fluid disposed in the shearing channel, and a pressure distribution system fluidly connected to each of the supply channel and the shearing channel to control at least relative pressures of the microbead precursor material and the carrier fluid. The supply channel includes a check valve adapted to be subjected to a bias pressure that is sufficient to close the check valve to flow of microbead precursor material when a supply pressure of the microbead precursor material is below a threshold pressure and is open to flow of the microbead precursor material when the supply pressure of the microbead precursor material is greater than the threshold pressure. An end of the supply channel opens into the shearing channel such that the microbead precursor material is sheared into droplets by the carrier fluid flowing through the shearing channel. A pressure of the carrier fluid is less than the bias pressure. The microbead precursor material and the carrier fluid are substantially immiscible.

    摘要翻译： 用于生产微珠的系统包括限定供应通道和剪切通道的微流体装置，设置在供应通道中的微珠前体材料，设置在剪切通道中的载体流体和流体连接到每个供应通道的压力分配系统 和剪切通道以控制微珠前体材料和载体流体的至少相对压力。 供应通道包括止回阀，该止回阀适于当微珠前体材料的供应压力低于阈值压力时足以关闭止回阀以流过微珠前体材料的偏压，并且对于 当微珠前体材料的供应压力大于阈值压力时，微珠前体材料。 供应通道的一端通向剪切通道，使得微珠前体材料被流过剪切通道的载体流体剪切成液滴。 载液的压力小于偏压。 微珠前体材料和载体流体基本上是不混溶的。

    FLUIDIC CHIPS HAVING SURFACE ENERGY TRAPS
    4.
    发明申请
    FLUIDIC CHIPS HAVING SURFACE ENERGY TRAPS 审中-公开
    具有表面能量的流体液体

    公开(公告)号：US20130303394A1

    公开(公告)日：2013-11-14

    申请号：US13856320

    申请日：2013-04-03

    申请人： Jeff Tza-Huei Wang ,  Yi Zhang

    发明人： Jeff Tza-Huei Wang ,  Yi Zhang

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6876 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2200/12 ,  B81B2201/058 ,  B81C1/00206 ,  C03C17/328

    摘要： A method of using a fluidic device includes providing a fluidic chip having a plurality of surface energy traps; depositing at least one fluid droplet on the fluidic chip, the fluid droplet including magnetic particles suspended therein; and moving at least a portion of the at least one fluid droplet across a surface of the fluidic chip by altering a magnetic interaction applied to the magnetic particles such that the at least one fluid droplet interacts with at least one of the plurality of surface energy traps.

    摘要翻译： 使用流体装置的方法包括提供具有多个表面能量阱的流体碎片; 在流体芯片上沉积至少一个流体液滴，所述流体液滴包括悬浮在其中的磁性颗粒; 以及通过改变施加到所述磁性颗粒的磁相互作用来移动所述至少一个流体液滴的至少一部分穿过所述流体芯片的表面，使得所述至少一个流体液滴与所述多个表面能量阱中的至少一个相互作用 。

    DNA INTEGRITY ASSAY (DIA) FOR CANCER DIAGNOSTICS, USING CONFOCAL FLUORESCENCE SPECTROSCOPY
    5.
    发明申请
    DNA INTEGRITY ASSAY (DIA) FOR CANCER DIAGNOSTICS, USING CONFOCAL FLUORESCENCE SPECTROSCOPY 有权
    DNA完整性测定（DIA）用于癌症诊断，使用共焦荧光光谱

    公开(公告)号：US20110171741A1

    公开(公告)日：2011-07-14

    申请号：US13120060

    申请日：2009-11-04

    申请人： Tza-Huei Wang ,  Kelvin J. Liu ,  Ie-Ming Shih

    发明人： Tza-Huei Wang ,  Kelvin J. Liu ,  Ie-Ming Shih

    IPC分类号： G01N33/52

    CPC分类号： C12Q1/6825 ,  C12Q2563/107 ,  C12Q2525/204

    摘要： The present invention relates, e.g., to a method for determining the size distribution of DNA molecules in a sample comprising cell-free nucleic acid, comprising labeling the DNA with a fluorescent dye in a stoichiometric manner, subjecting the DNA to molecular spectroscopy (e.g., cylindrical illumination confocal spectroscopy), analyzing suitable fluorescent burst parameters of the labeled DNA, and conducting single molecule DNA integrity analysis of the labeled DNA molecules in the sample. In one embodiment of the invention, the method is used as a diagnostic method for detecting cancer.

    摘要翻译： 本发明涉及例如用于确定包含无细胞核酸的样品中DNA分子的大小分布的方法，包括以化学计量方式用荧光染料标记DNA，对DNA进行分子光谱学（例如， 圆柱形照明共焦光谱），分析标记DNA的合适的荧光突变参数，并对样品中标记的DNA分子进行单分子DNA完整性分析。 在本发明的一个实施方案中，该方法用作检测癌症的诊断方法。

    Electrochemical detection of mismatch nucleic acids
    6.
    发明授权
    Electrochemical detection of mismatch nucleic acids 有权
    错配核酸的电化学检测

    公开(公告)号：US07291457B2

    公开(公告)日：2007-11-06

    申请号：US10177537

    申请日：2002-06-21

    申请人： Jeffrey F. Miller ,  Jun Huang ,  Tza-Huei Wang ,  Chih-Ming Ho ,  Minghsun Liu

    发明人： Jeffrey F. Miller ,  Jun Huang ,  Tza-Huei Wang ,  Chih-Ming Ho ,  Minghsun Liu

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6816 ,  C12Q1/6827 ,  C12Q2565/607 ,  C12Q2537/125 ,  C12Q2525/301 ,  C12Q2563/113

    摘要： A method and system for specifically detecting perfectly matched probe-target hybrids by annealing a hairpin-based probe to a nucleic acid target under conditions favoring the formation of perfectly matched targets over mismatched targets, and electrochemically detecting the annealed probe. Sensitivity of detection can be enhanced by catalyzed reporter deposition of probe signal prior to electrochemical detection. Specificity can be enhanced by including hairpin-based competitor probes in the annealing step. The detection assay is particularly applicable to typing single nucleotide polymorphisms by DNA sandwich hybridization.

    摘要翻译： 一种用于特异性检测完全匹配的探针 - 靶杂交体的方法和系统，所述方法和系统通过在有助于在失配靶上形成完全匹配的靶的条件下将发夹基探针退火至核酸靶，以及电化学检测退火的探针。 在电化学检测之前，探测信号的催化报道沉积可以提高检测灵敏度。 通过在退火步骤中包含基于发夹的竞争者探针可以提高特异性。 检测方法特别适用于通过DNA夹心杂交分型单核苷酸多态性。

    Biosensors and methods for their use
    8.
    发明申请
    Biosensors and methods for their use 审中-公开
    生物传感器及其使用方法

    公开(公告)号：US20070031961A1

    公开(公告)日：2007-02-08

    申请号：US11501424

    申请日：2006-08-09

    申请人： Chih-Ming Ho ,  Tza-Huei Wang

    发明人： Chih-Ming Ho ,  Tza-Huei Wang

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： B01L3/5027 ,  B01J2219/00317 ,  B01J2219/00511 ,  B01J2219/00576 ,  B01J2219/00605 ,  B01J2219/00653 ,  B01J2219/00666 ,  B01J2219/00702 ,  B01L3/502707 ,  B01L2300/168 ,  C12Q1/6825 ,  C40B60/14 ,  G01N21/0303 ,  G01N21/05 ,  G01N21/6428 ,  G01N21/645 ,  G01N27/4145 ,  G01N27/447 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2021/6463 ,  G01N2021/6476 ,  G01N2021/6482

    摘要： The invention disclosed herein provides biosensors and methods which increase the sensitivity of assays of optically labelled molecules fluorescently tagged polypeptides and polynucleotides while decreasing the sample volume required for detection. By integrating reflective sidewalls into the receptacles used in such assays, the signal-to-noise ratio of the optical signal is increased significantly. Typically the receptacles are microchannels. In addition, the geometry of the receptacles can be controlled to further optimize the signal-to-noise ratio of the optical signal. The invention disclosed herein further provides methods and devices involving integrated electronics, wherein an element such as a diode, a transistor, an integrated circuit etc., is integrated with a bio-reactor/channel in order to facilitate the detection or fabrication of bio-materials.

    摘要翻译： 本文公开的本发明提供了生物传感器和方法，其增加光学标记分子荧光标记的多肽和多核苷酸的测定的灵敏度，同时降低检测所需的样品体积。 通过将反射侧壁集成在这种测定中使用的插座中，光信号的信噪比显着增加。 通常，插座是微通道。 此外，可以控制插座的几何形状以进一步优化光信号的信噪比。 本文公开的本发明进一步提供了涉及集成电子器件的方法和装置，其中诸如二极管，晶体管，集成电路等的元件与生物反应器/通道集成，以便于生物反应器/通道的检测或制造， 材料

    Cylindrical illumination confocal spectroscopy system
    10.
    发明授权
    Cylindrical illumination confocal spectroscopy system 有权
    圆柱照明共焦光谱系统

    公开(公告)号：US08248609B2

    公开(公告)日：2012-08-21

    申请号：US12612300

    申请日：2009-11-04

    申请人： Tza-Huei Wang ,  Kelvin J. Liu

    发明人： Tza-Huei Wang ,  Kelvin J. Liu

    IPC分类号： G01J3/46

    CPC分类号： G01J3/02 ,  G01J3/0208 ,  G01J3/0229 ,  G01J3/024 ,  G01J3/10 ,  G01J3/44

    摘要： A cylindrical illumination confocal spectroscopy system has a fluidic device having a fluid channel defined therein, an objective lens unit arranged proximate the fluidic device, an illumination system in optical communication with the objective lens unit to provide light to illuminate a sample through the objective lens unit, and a detection system in optical communication with the objective lens unit to receive at least a portion of light that passes through the objective lens unit from the sample. The illumination system includes a beam-shaping lens unit constructed and arranged to provide a substantially planar illumination beam that subtends across, and is longer than, a lateral dimension of the fluid channel.

    摘要翻译： 圆柱形照明共焦光谱系统具有其中限定有流体通道的流体装置，靠近流体装置布置的物镜单元，与物镜单元光学连通的照明系统，以提供通过物镜单元照射样品的光 以及与所述物镜单元光学通信以从所述样品接收经过所述物镜单元的至少一部分光的检测系统。 照明系统包括光束整形透镜单元，其被构造和布置成提供对准流体通道的横向尺寸并且比其更长的基本上平面的照明光束。