发明授权
- 专利标题: Kits and methods for generating 5′ capped RNA
- 专利标题(中): 用于产生5'封端RNA的试剂盒和方法
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申请号: US14185384申请日: 2014-02-20
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公开(公告)号: US08846348B2公开(公告)日: 2014-09-30
- 发明人: Jerome Jendrisak , Ronald Meis , Gary Dahl
- 申请人: CellScript, LLC
- 申请人地址: US WI Madison
- 专利权人: CellScript, LLC
- 当前专利权人: CellScript, LLC
- 当前专利权人地址: US WI Madison
- 代理机构: Casimir Jones S.C.
- 主分类号: C12P19/34
- IPC分类号: C12P19/34 ; C12P21/06 ; C12N15/10
摘要:
The present invention relates to kits and methods for efficiently generating 5′ capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production of 5′-capped RNA having a modified cap nucleotide and for in vitro or in vivo production of polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate, and methods and kits for using a capping enzyme system and modified cap nucleotides for labeling uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate with detectable dye or enzyme moieties.
公开/授权文献
- US20140221248A1 KITS AND METHODS FOR GENERATING 5' CAPPED RNA 公开/授权日:2014-08-07
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