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    KITS AND METHODS FOR GENERATING 5' CAPPED RNA
    3.
    发明申请
    KITS AND METHODS FOR GENERATING 5' CAPPED RNA 有权

    公开(公告)号：US20140363876A1

    公开(公告)日：2014-12-11

    申请号：US14471649

    申请日：2014-08-28

    申请人： CELLSCRIPT, LLC

    发明人： Jerome Jendrisak ,  Ronald Meis ,  Gary Dahl

    IPC分类号： C12P19/34

    CPC分类号： C12P19/34 ,  C12N9/1007 ,  C12N9/1241 ,  C12N15/1072 ,  C12N15/1075 ,  C12N15/1096 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/317 ,  C12N2320/51 ,  C12Y201/01056 ,  C12Y201/01057 ,  C12Y207/07019 ,  C12Y207/0705

    摘要： The present invention relates to kits and methods for efficiently generating 5′ capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production of 5′-capped RNA having a modified cap nucleotide and for in vitro or in vivo production of polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate, and methods and kits for using a capping enzyme system and modified cap nucleotides for labeling uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate with detectable dye or enzyme moieties.

    KITS AND METHODS FOR GENERATING 5' CAPPED RNA
    7.
    发明申请
    KITS AND METHODS FOR GENERATING 5' CAPPED RNA 有权
    用于产生5'CAPPED RNA的基因座和方法

    公开(公告)号：US20140221248A1

    公开(公告)日：2014-08-07

    申请号：US14185384

    申请日：2014-02-20

    申请人： CELLSCRIPT, LLC

    发明人： Jerome Jendrisak ,  Ronald Meis ,  Gary Dahl

    IPC分类号： C12N15/10

    CPC分类号： C12P19/34 ,  C12N9/1007 ,  C12N9/1241 ,  C12N15/1072 ,  C12N15/1075 ,  C12N15/1096 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/317 ,  C12N2320/51 ,  C12Y201/01056 ,  C12Y201/01057 ,  C12Y207/07019 ,  C12Y207/0705

    摘要： The present invention relates to kits and methods for efficiently generating 5′ capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production of 5′-capped RNA having a modified cap nucleotide and for in vitro or in vivo production of polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate, and methods and kits for using a capping enzyme system and modified cap nucleotides for labeling uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate with detectable dye or enzyme moieties.

    摘要翻译： 本发明涉及用于有效产生具有修饰的帽核苷酸的5'封端RNA并用于这种经修饰的核苷酸封端的RNA分子的试剂盒和方法。 特别地，本发明提供了使用修饰的帽核苷酸和封端酶系统（例如痘病毒覆盖酶）对RNA进行封端的试剂盒和方法。 本发明用于通过体外或体内翻译这些经修饰的核苷酸加帽的RNA来体外生产具有修饰的帽核苷酸的5'-加帽的RNA，以及体外或体内产生多肽。 本发明还提供用于捕获或分离包含初级RNA转录物或具有5'-二磷酸的RNA的未封端RNA的方法和试剂盒，以及使用封端酶系统和修饰的帽核苷酸用于标记包含初级RNA转录物或RNA的未加帽RNA的方法和试剂盒 具有可检测的染料或酶部分的5'-二磷酸酯。