发明授权
- 专利标题: Phage display using cotranslational translocation of fusion polypeptides
- 专利标题(中): 噬菌体展示使用融合多态的转译易位
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申请号: US11988389申请日: 2006-06-30
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公开(公告)号: US08846577B2公开(公告)日: 2014-09-30
- 发明人: Daniel Steiner , Patrik Forrer , Michael T. Stumpp , Andreas Plückthun
- 申请人: Daniel Steiner , Patrik Forrer , Michael T. Stumpp , Andreas Plückthun
- 申请人地址: CH Zurich
- 专利权人: University of Zurich
- 当前专利权人: University of Zurich
- 当前专利权人地址: CH Zurich
- 代理机构: Wenderoth, Lind & Ponack, L.L.P.
- 优先权: EP05106236 20050708
- 国际申请: PCT/EP2006/063729 WO 20060630
- 国际公布: WO2007/006665 WO 20070118
- 主分类号: C40B50/06
- IPC分类号: C40B50/06 ; C12N15/10
摘要:
The present invention relates to a filamentous phage display method wherein the polypeptides of interest displayed on the phage particle are cotranslationally translocated across the cytoplasmic membrane of Gram-negative bacteria based on the signal recognition particle pathway. This method is particularly suitable for polypeptides, which are known to be difficult to display on phages, and for proteins of cDNA libraries and other combinatorial libraries, in particular when derived from very fast folding, stable protein scaffolds. The invention further relates to phage or phagemid vectors useful in the method comprising a gene construct coding for a fusion polypeptide comprising the polypeptide to be displayed on the phage particle and an N-terminal signal sequence promoting cotranslational translocation.
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