发明授权
- 专利标题: Detection of multiple nucleic acid sequences in a reaction cartridge
- 专利标题(中): 检测反应筒中的多个核酸序列
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申请号: US13318424申请日: 2010-05-04
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公开(公告)号: US08852863B2公开(公告)日: 2014-10-07
- 发明人: Thomas Rothmann , Holger Engel , Ralf Himmelreich , Andy Wende , Rainer Dahlke
- 申请人: Thomas Rothmann , Holger Engel , Ralf Himmelreich , Andy Wende , Rainer Dahlke
- 申请人地址: DE Hilden
- 专利权人: Qiagen GmbH
- 当前专利权人: Qiagen GmbH
- 当前专利权人地址: DE Hilden
- 代理机构: Fanelli Haag & Kilger PLLC
- 优先权: EP09159429 20090505
- 国际申请: PCT/EP2010/056030 WO 20100504
- 国际公布: WO2010/128041 WO 20101111
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12P19/34
摘要:
The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set and performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.
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