发明授权
US08883504B2 Cell differentiation suppressing agent, method of culturing cells using the same, culture solution, and cultured cell line
有权
细胞分化抑制剂,使用其的培养细胞的培养方法,培养液和培养细胞系
- 专利标题: Cell differentiation suppressing agent, method of culturing cells using the same, culture solution, and cultured cell line
- 专利标题(中): 细胞分化抑制剂,使用其的培养细胞的培养方法,培养液和培养细胞系
-
申请号: US12233252申请日: 2008-09-18
-
公开(公告)号: US08883504B2公开(公告)日: 2014-11-11
- 发明人: Tomoyuki Miyabayashi , Masashi Yamamoto
- 申请人: Tomoyuki Miyabayashi , Masashi Yamamoto
- 申请人地址: JP Osaka-Shi
- 专利权人: Asahi Kasei Kabushiki Kaisha
- 当前专利权人: Asahi Kasei Kabushiki Kaisha
- 当前专利权人地址: JP Osaka-Shi
- 代理机构: Birch, Stewart, Kolasch & Birch, LLP
- 优先权: JP2003-185398 20030627; JP2003-353870 20031014
- 主分类号: C12N5/00
- IPC分类号: C12N5/00 ; C07D217/16 ; A61K31/47 ; A61K31/404 ; A61K31/655 ; A61K31/472
摘要:
The object of the present invention is to provide a differentiation inhibiting agent which allows culture of a stem cell or an embryonic stem cell in an undifferentiated state without use of any feeder cell, a method for culturing using the same, a cell culture liquid using the same, and a cell prepared by culturing using this differentiation inhibiting agent. The present invention provides a differentiation inhibiting agent which comprises a low molecular weight compound, especially a tetrahydroisoquinoline derivative, as an active ingredient; a method for safely culturing a stem cell in large scale in undifferentiated state in the absence of feeder cell which comprises culturing a stem cell by using a tetrahydroisoquinoline derivative; a culture liquid for stem cells comprising a tetrahydroisoquinoline derivative; and a cell which is obtained by culture using a tetrahydroisoquinoline derivative as a differentiation inhibiting agent.
公开/授权文献
信息查询