发明授权
US08895787B2 Compositions comprising a dehalogenase substrate and a radionuclide and methods of use
有权
包含脱卤素酶底物和放射性核素的组合物及其使用方法
- 专利标题： Compositions comprising a dehalogenase substrate and a radionuclide and methods of use
- 专利标题（中）： 包含脱卤素酶底物和放射性核素的组合物及其使用方法
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申请号： US13450233申请日： 2012-04-18
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公开(公告)号： US08895787B2公开(公告)日： 2014-11-25
- 发明人: Keith V. Wood , Georgyi V. Los , Robert F. Bulleit , Dieter Klaubert , Mark McDougall , Chad Zimprich
- 申请人： Keith V. Wood , Georgyi V. Los , Robert F. Bulleit , Dieter Klaubert , Mark McDougall , Chad Zimprich
- 申请人地址： US WI Madison
- 专利权人： Promega Corporation
- 当前专利权人： Promega Corporation
- 当前专利权人地址： US WI Madison
- 代理机构： Casimir Jones SC
- 主分类号： C07C19/00
- IPC分类号： C07C19/00 ; C07C21/00 ; G01N33/53 ; C12Q1/34 ; C12N9/24
摘要：
A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.