A cut-off wavelength measuring method according to the present invention includes: preparing a single mode fiber as a reference fiber; preparing a measurement target fiber; adjusting the length of the single mode fiber such that the length of the single mode fiber is longer than the that of the measurement target fiber at the time of measuring power of transmission light and the reference fiber propagates only light of a base mode at a predicted cut-off wavelength of the measurement target fiber; measuring wavelength dependence of power of light transmitted through the reference fiber and wavelength dependence of power of light transmitted through the measurement target fiber; and calculating a cut-off wavelength of the measurement target fiber based on wavelength dependence represented as the ratio of the power of transmission light transmitted through the measurement target fiber to the power of light transmitted through the reference fiber.