A method for transmitting a sounding reference signal from a user equipment in a MIMO antenna wireless communication system. The method includes receiving sounding reference signal setup information from a base station, the sounding reference signal setup information including an initial cyclic shift value nSRScs and an initial transmissionComb parameter value kTC; setting an interval between cyclic shift values corresponding to each antenna port based on the initial cyclic shift value, to reach a maximum interval; setting a transmissionComb parameter value corresponding to a specific one of the antenna ports to a value different from the initial transmissionComb parameter value if the initial cyclic shift value is a previously set value and the number of antenna ports is 4; and transmitting the sounding reference signal to the base station through each antenna port by using the set cyclic shift value and transmissionComb parameter value.