Invention Grant
US08993335B2 Methods for detection and analysis of polynucleotide-binding protein interactions
有权
检测和分析多核苷酸结合蛋白相互作用的方法
- Patent Title: Methods for detection and analysis of polynucleotide-binding protein interactions
- Patent Title (中): 检测和分析多核苷酸结合蛋白相互作用的方法
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Application No.: US10779412Application Date: 2004-02-13
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Publication No.: US08993335B2Publication Date: 2015-03-31
- Inventor: Guillermo C. Bazan , Shu Wang , Bin Liu
- Applicant: Guillermo C. Bazan , Shu Wang , Bin Liu
- Applicant Address: US CA Oakland
- Assignee: The Regents of the University of California
- Current Assignee: The Regents of the University of California
- Current Assignee Address: US CA Oakland
- Agency: Bozicevic, Field & Francis LLP
- Agent Bret E. Field
- Main IPC: G01N33/00
- IPC: G01N33/00 ; C12Q1/68 ; C07K1/00 ; C07H21/02
Abstract:
Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor PBP that can bind to the target polynucleotide. The sensor PBP comprises a signaling chromophore to absorb energy from the excited multichromophore and emit light in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.
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