发明授权
- 专利标题: Selection method of induced pluripotent stem cells
- 专利标题(中): 诱导多能干细胞的选择方法
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申请号: US13393528申请日: 2010-09-01
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公开(公告)号: US09005976B2公开(公告)日: 2015-04-14
- 发明人: Shinya Yamanaka , Takayuki Tanaka
- 申请人: Shinya Yamanaka , Takayuki Tanaka
- 申请人地址: JP Kyoto
- 专利权人: Kyoto University
- 当前专利权人: Kyoto University
- 当前专利权人地址: JP Kyoto
- 代理机构: Knobbe, Martens, Olson & Bear LLP
- 国际申请: PCT/JP2010/065357 WO 20100901
- 国际公布: WO2011/027901 WO 20110310
- 主分类号: C12N15/00
- IPC分类号: C12N15/00 ; C12Q1/68
摘要:
The present invention relates to a method for selecting induced pluripotent stem (iPS) cells. More particularly, the present invention provides: a method for selecting an iPS cell, comprising the steps of: (1a) measuring the expression level of an exogenous nuclear reprogramming gene(s) in a test iPS cell; and (2a) selecting an iPS cell in which the expression level(s) of an exogenous nuclear reprogramming gene(s) is/are less than or equal to the expression level(s) in control iPS cells; and a method for selecting an iPS cell, comprising the steps of: (1b) measuring the expression level of an exogenous nuclear reprogramming gene(s) and the sum the expression levels of the exogenous nuclear reprogramming gene(s) and the corresponding endogenous gene iPS cell; and (2b) selecting an iPS cell in which the ratio of the expression level of an exogenous nuclear reprogramming gene(s) relative to the sum of the expression levels of the exogenous transgene(s) and the corresponding endogenous gene(s) is less than 1 to the ratio in the control iPS cell.
公开/授权文献
- US20120156684A1 SELECTION METHOD OF INDUCED PLURIPOTENT STEM CELLS 公开/授权日:2012-06-21
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