A method of manufacturing a touch screen substrate includes forming a sensing electrode on a substrate, spraying a first ink and a second ink on the substrate including the sensing electrode, where the first ink includes a first solvent and insulation balls having a first diameter and the second ink includes a second solvent and conductive balls having a second diameter smaller than the first diameter, and hardening the first and second inks on the substrate, to fix the insulation balls on the substrate and the conductive balls on the sensing electrode formed on the substrate.