发明授权
- 专利标题： RNA in situ hybridization
- 专利标题（中）： RNA原位杂交
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申请号： US12993338申请日： 2009-05-19
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公开(公告)号： US09051610B2公开(公告)日： 2015-06-09
- 发明人: Hirofumi Doi , Masahiro Matsuoka , Tatsuhiro Nakano , Chizuru Nagayoshi
- 申请人： Hirofumi Doi , Masahiro Matsuoka , Tatsuhiro Nakano , Chizuru Nagayoshi
- 申请人地址： JP Chiba
- 专利权人： CELISH FD, INC.
- 当前专利权人： CELISH FD, INC.
- 当前专利权人地址： JP Chiba
- 代理机构： Wenderoth, Lind & Ponack, L.L.P.
- 优先权： JP2008-131302 20080519
- 国际申请： PCT/JP2009/059219 WO 20090519
- 国际公布： WO2009/142214 WO 20091126
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; G01N33/53 ; C12M1/34 ; G01N21/64 ; G01N31/22 ; C07H21/04 ; C07H21/02
摘要：
A method for identifying the presence of a target gene mRNA is provided, which involves hybridizing one or more oligonucleic acid probes with the target gene mRNA expressed in a tissue sample, and detecting a low-molecular-weight compound label added to at least one of the bases of the oligonucleic acid probes. The oligonucleic acid probes are contacted with the tissue sample for hybridization with the target gene mRNA after pretreating (prehybridizing) one or more dummy oligonucleic acids with the tissue sample, or a mixture of the oligonucleic acid probes and the dummy oligonucleic acids is contacted with the sample tissue to hybridize the oligonucleic acid probes with the target gene mRNA.
公开/授权文献
- US20110183331A1 RNA IN SITU HYBRIDIZATION 公开/授权日：2011-07-28