Electronic devices may include displays having backlight structures and display layers. The display layers may include alignment features. The backlight structure may include alignment features. The alignment features on the backlight structures may include transparent portions of the backlight structures. The alignment features on the display layers may include alignment marks that are visible through the transparent portions of the backlight structures. The transparent portions of the backlight structures may include openings that extend from a first surface of the backlight structures to an opposing second surface of the backlight structures. The openings may be filled by transparent members formed from plastic or glass. The transparent members may include additional alignment marks. The transparent members may include lensing portions that magnify the alignment marks on the display layers when viewed through the lensing portions.