The present invention provides a connection structure for panel and frame and holding member thereof. The connection structure includes a panel and a frame supporting the panel. The panel and the frame use a plurality of holding members to fix and hold. The holding member includes a presser and a connection part extending from one side of the presser. The connection part includes a first extension part and a second extension part. The first extension part and the second extension part connect a corresponding connection element on the side of the frame. As such, the present invention saves the front frame and fixes a liquid crystal panel and a frame of a liquid crystal module to reduce the transportation risk, protect the liquid crystal panel from damage, simplify the overall assembly structure of the liquid crystal module and reduce the assembly cost.