发明授权
US09121010B1 Continuous porcine kidney cell line constitutively expressing bovine αVβ6 integrin with increased susceptibility to foot and mouth disease virus
有权
连续猪肾细胞系组成型表达牛αV＆bgr; 6整合素，增加对口蹄疫病毒的易感性
- 专利标题： Continuous porcine kidney cell line constitutively expressing bovine αVβ6 integrin with increased susceptibility to foot and mouth disease virus
- 专利标题（中）： 连续猪肾细胞系组成型表达牛αV＆bgr; 6整合素，增加对口蹄疫病毒的易感性
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申请号： US13662104申请日： 2012-10-26
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公开(公告)号： US09121010B1公开(公告)日： 2015-09-01
- 发明人: Michael A Larocco , Barry Baxt , Hernando Duque
- 申请人： The United States of America, as Represented by the Secretary of Agriculture
- 申请人地址： US DC Washington
- 专利权人： The United States of America, as represented by The Secretary of Agriculture
- 当前专利权人： The United States of America, as represented by The Secretary of Agriculture
- 当前专利权人地址： US DC Washington
- 代理商 John D. Fado; Evelyn M. Rabin
- 主分类号： C12N15/85
- IPC分类号： C12N15/85 ; G01N33/53 ; C12N5/071
摘要：
Foot and out disease virus (FMDV) is worldwide problem. Rapid isolation, serotyping and vaccine matching of FMDV from infected animals is critical to enable the implementation of effective vaccination programs and to stop the spread of infection during outbreaks. Current virus isolation protocols use primary cells, known to be susceptible to FMDV, or baby hamster kidney cells (BHK-21) and other cell lines that are not highly sensitive to some strains of FMDV. The αVβ6 integrin is a principal receptor for FMDV. We therefore transduced the porcine kidney cell line, LFBK, to stably express both the αV and β6 bovine integrin subunits. The LFBK-αVβ6 cell line showed both β6 expression and enhanced susceptibility to FMDV infection for at least 100 cell passages. LFBK-αVβ6 cells are highly sensitive for detecting all serotypes of FMDV from experimentally infected animals, including the porcinophillic strain O/TAW/97 and are thus a sensitive tool for FMDV isolation.
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