A method and an apparatus for transmitting and receiving a broadcasting stream for providing a digital broadcasting service including a subtitle service are provided. A method of transmitting a data stream includes: generating a subtitle stream including basic viewpoint subtitle data and additional viewpoint subtitle data to reproduce a three-dimensional (3D) subtitle on a video screen on which video data is reproduced; determining 3D subtitle reproduction information for forming the 3D subtitle on the video screen based on the basic viewpoint subtitle data; and outputting a data stream by multiplexing a video stream including the video data, the 3D subtitle reproduction information, and the subtitle stream.