A display device having a plurality of pixel units and a manufacturing method of the same are provided. The display device includes a first substrate assembly, a second substrate assembly, a liquid crystal mixture, and a pillared polymer network. The first substrate assembly includes a first substrate and a first electrode layer disposed on the first substrate. The second substrate assembly includes a second substrate. The liquid crystal mixture is disposed between the first and second substrate assemblies. The pillared polymer network is disposed between the first and second substrate assemblies and has a first end and second end. The first end abuts against the first substrate assembly and is disposed correspondingly to the first electrode layer. The second end abuts against the second substrate assembly. Each of the pixel units includes the pillared polymer network.