A method and apparatus are provided for transmitting and receiving an uplink Sounding Reference Signal (SRS). The method includes receiving index information for an SRS; determining an SRS offset and an SRS period, based on the index information; and transmitting the SRS, based on the SRS offset and the SRS period. If the index information includes an integer from 10 to 644, the SRS period is selected among 5 ms, 10 ms, 20 ms, 40 ms, 80 ms, 160 ms, and 320 ms. If the index information includes an integer from 0 to 9, the SRS is transmitted twice in a period of 5 ms, and the SRS offset indicated by the index information is based on a predetermined table IndexOffset 00, 1 10, 2 21, 2 30, 3 50, 4 61, 4 72, 3 82, 4 9 3, 4.