A method and apparatus are provided for transmitting and receiving an SRS. The method includes determining a number of SC-FDMA symbols in an UpPTS; receiving index information for an SRS; determining an SRS offset and an SRS period, based on the index information; and transmitting the SRS, based on the SRS offset and the SRS period. If the index information includes an integer from 0 to 9, the SRS is transmitted twice in a period of 5 ms, and if the UpPTS includes two SC-FDMA symbols, a first symbol is indicated by SRS offset 0 and a second symbol is indicated by SRS offset 1, but if the UpPTS includes one SC-FDMA symbol, the first symbol is indicated by the SRS offset 1. If the index information includes an integer from 10 to 644, the SRS period is selected among 5 ms, 10 ms, 20 ms, 40 ms, 80 ms, 160 ms, and 320 ms.