A touch display substrate includes a first data line, a first gate line, a first pixel electrode, a second gate line, a second pixel electrode, a sensor data line and a first sensor electrode. The first data line extends along a first direction. The first gate line extends along a second direction. The first pixel electrode is electrically connected to the first data line and the first gate line. The second gate line is substantially parallel with the first gate line. The second pixel electrode is adjacent to the first pixel electrode and electrically connected to the first data line and the second gate line. The sensor data line is adjacent to the second pixel electrode and substantially parallel to the first data line. The first sensor electrode is electrically connected to the sensor data line.