Systems and methods for multispectral imaging are disclosed. The multispectral imaging system can include a near infrared (NIR) imaging sensor and a visible imaging sensor. The disclosed systems and methods can be implemented to de-noise a visible light image using a gradient scale map generated from gradient vectors in the visible light image and a NIR image. The gradient scale map may be used to determine the amount of de-noising guidance applied from the NIR image to the visible light image on a pixel-by-pixel basis.