发明授权
US09175327B2 Composition for synthesizing protein with reduced lipopolysaccharide contamination, method for producing protein using said composition
有权
用于合成具有减少的脂多糖污染的蛋白质的组合物，使用所述组合物生产蛋白质的方法
- 专利标题： Composition for synthesizing protein with reduced lipopolysaccharide contamination, method for producing protein using said composition
- 专利标题（中）： 用于合成具有减少的脂多糖污染的蛋白质的组合物，使用所述组合物生产蛋白质的方法
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申请号： US13100050申请日： 2011-05-03
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公开(公告)号： US09175327B2公开(公告)日： 2015-11-03
- 发明人: Takashi Kanamori , Yuki Hayami , Kanehisa Kojoh , Takuya Ueda , Kumiko Tsuihiji , Tomoe Fuse , Mikiko Nakamura , Shizue Kato
- 申请人： Takashi Kanamori , Yuki Hayami , Kanehisa Kojoh , Takuya Ueda , Kumiko Tsuihiji , Tomoe Fuse , Mikiko Nakamura , Shizue Kato
- 申请人地址： JP Kashiwa JP Tokyo
- 专利权人： GeneFrontier Corporation,The University of Tokyo
- 当前专利权人： GeneFrontier Corporation,The University of Tokyo
- 当前专利权人地址： JP Kashiwa JP Tokyo
- 代理机构： Leydig, Voit & Mayer, Ltd.
- 优先权： JP2011-053547 20110310
- 主分类号： C12P21/02
- IPC分类号： C12P21/02 ; C12N15/10
摘要：
According to the present invention, a composition possessing cell-free protein synthesis activity with reduced contaminating lipopolysaccharide, and a method for producing a protein using the same are provided. When ribosome display is performed using the composition and method for protein production of the present invention, the background that is caused by non-specific binding is reduced, so that a nucleic acid that encodes the desired polypeptide can be selected with high accuracy and high efficiency.
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