Invention Grant
- Patent Title: In vitro evolution in microfluidic systems
- Patent Title (中): 微流体系统体外进化
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Application No.: US13692615Application Date: 2012-12-03
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Publication No.: US09186643B2Publication Date: 2015-11-17
- Inventor: Andrew David Griffiths , David A. Weitz , Darren R. Link , Keunho Ahn , Jerome Bibette
- Applicant: Medical Research Council , President and Fellows of Harvard College
- Applicant Address: GB London US MA Cambridge
- Assignee: Medical Research Council,President and Fellows of Harvard College
- Current Assignee: Medical Research Council,President and Fellows of Harvard College
- Current Assignee Address: GB London US MA Cambridge
- Agency: Brown Rudnick LLP
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P21/06 ; C12M1/00 ; G01N15/06 ; G01N33/52 ; C40B10/00 ; C07H21/02 ; B01J19/00 ; B01F3/08 ; B01F5/06 ; B01F13/00 ; B01L3/00 ; C12N15/10 ; C12P21/00
Abstract:
The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalizing genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
Public/Granted literature
- US20130157872A1 IN VITRO EVOLUTION IN MICROFLUIDIC SYSTEMS Public/Granted day:2013-06-20
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