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公开(公告)号：US10371699B2
公开(公告)日：2019-08-06
申请号：US15331445
申请日：2016-10-21
IPC分类号： B01L3/00 , G01N33/543 , B01F3/08 , B01F5/02 , B01F5/06 , B01F13/00 , G01N33/50 , B01J19/00 , C12Q1/42 , G01N15/14 , G01N21/64 , G01N33/573 , C40B50/08
摘要： The invention describes a method for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, comprising the steps of: a) compartmentalizing the compounds into microcapsules together with the target, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule; and b) identifying the compound which binds to or modulates the activity of the target; wherein at least one step is performed under microfluidic control. The invention enables the screening of large repertoires of molecules which can serve as leads for drug development.
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公开(公告)号：US20180272299A1
公开(公告)日：2018-09-27
申请号：US15922292
申请日：2018-03-15
IPC分类号： B01F13/00 , B01F5/06 , B01J19/00 , B01L3/00 , B82Y10/00 , B82Y30/00 , C07K1/04 , C40B40/06 , C40B40/10 , C40B50/08 , B01F3/08 , G01N35/10
CPC分类号： C40B40/08 , B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0071 , B01F13/0076 , B01J13/025 , B01J19/0046 , B01J2219/00497 , B01J2219/005 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00599 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01J2219/00743 , B01J2219/00853 , B01J2219/0086 , B01J2219/00889 , B01L3/5025 , B01L3/5027 , B01L3/50273 , B01L3/502746 , B01L3/502761 , B01L3/502784 , B01L2200/0605 , B01L2200/0673 , B01L2300/0864 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , B01L2400/084 , B82Y10/00 , B82Y30/00 , C07K1/047 , C12N15/1096 , C12Q2600/16 , C40B40/06 , C40B40/10 , C40B50/08 , G01N2035/1034 , H05K999/99
摘要： The invention describes a method for the synthesis of compounds comprising the steps of: (a) compartmentalising two or more sets of primary compounds into microcapsules; such that a proportion of the microcapsules contains two or more compounds; and (b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; wherein one or both of steps (a) and (b) is performed under microfluidic control; preferably electronic microfluidic control The invention further allows for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, and which is co-compartmentalised into the microcapsules.
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公开(公告)号：US20160186256A1
公开(公告)日：2016-06-30
申请号：US15012209
申请日：2016-02-01
IPC分类号： C12Q1/68
CPC分类号： B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/0052 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
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公开(公告)号：US09925501B2
公开(公告)日：2018-03-27
申请号：US15012209
申请日：2016-02-01
CPC分类号： B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/0052 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
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公开(公告)号：US20170282133A1
公开(公告)日：2017-10-05
申请号：US15588210
申请日：2017-05-05
CPC分类号： B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/0052 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
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公开(公告)号：US20170102381A1
公开(公告)日：2017-04-13
申请号：US15331445
申请日：2016-10-21
IPC分类号： G01N33/543 , B01F3/08 , G01N21/64 , C12Q1/42 , B01J19/00 , G01N15/14 , B01F13/00 , G01N33/573
CPC分类号： G01N33/5432 , B01F3/0807 , B01F5/0256 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01F2003/0834 , B01F2003/0842 , B01J19/0046 , B01J2219/00576 , B01J2219/00596 , B01J2219/00599 , B01J2219/00657 , B01J2219/00664 , B01J2219/00666 , B01J2219/00702 , B01L3/502761 , B01L3/502784 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12Q1/42 , C12Q2563/159 , C12Q2565/119 , C40B50/08 , G01N15/14 , G01N21/6428 , G01N33/5008 , G01N33/5044 , G01N33/573 , G01N2015/149 , G01N2021/6439 , G01N2333/916 , G01N2500/04 , G01N2500/10
摘要： The invention describes a method for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, comprising the steps of: a) compartmentalising the compounds into microcapsules together with the target, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule; and b) identifying the compound which binds to or modulates the activity of the target; wherein at least one step is performed under microfluidic control. The invention enables the screening of large repertoires of molecules which can serve as leads for drug development.
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公开(公告)号：US08871444B2
公开(公告)日：2014-10-28
申请号：US13693356
申请日：2012-12-04
IPC分类号： C12Q1/68 , G01N33/53 , C12P19/34 , C12M1/00 , G01N15/06 , C07H21/04 , B01L3/00 , B01F13/00 , B01J19/00 , B01F5/06 , C12N15/10 , B01F3/08 , C12P21/00
CPC分类号： B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/0052 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalizing genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
摘要翻译： 本发明描述了一种用于分离编码具有所需活性的基因产物的一种或多种遗传元件的方法，包括以下步骤：（a）将遗传元件分隔成微胶囊; 和（b）分选表达具有所需活性的基因产物的遗传元件; 其中至少一个步骤处于微流控制之下。 本发明能够通过本发明方法的重复诱变和迭代应用来体外进化核酸和蛋白质。
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公开(公告)号：US20180272294A1
公开(公告)日：2018-09-27
申请号：US15912033
申请日：2018-03-05
CPC分类号： B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/0052 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
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公开(公告)号：US09919277B2
公开(公告)日：2018-03-20
申请号：US15588210
申请日：2017-05-05
CPC分类号： B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/0052 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
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公开(公告)号：US20190024261A1
公开(公告)日：2019-01-24
申请号：US15835738
申请日：2017-12-08
摘要： The invention describes a method for the synthesis of compounds comprising the steps of: (a) compartmentalizing two or more sets of primary compounds into microcapsules; such that a proportion of the microcapsules contains two or more compounds; and (b) forming secondary compounds in the microcapsules by chemical reaction between primary compounds from different sets; wherein one or both of steps (a) and (b) is performed under microfluidic control; preferably electronic microfluidic control. The invention further allows for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, and which is co-compartmentalized into the microcapsules.
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