发明授权
- 专利标题: Replication deficient influenza virus for the expression of heterologous sequences
- 专利标题(中): 复制缺陷型流感病毒用于表达异源序列
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申请号: US14049601申请日: 2013-10-09
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公开(公告)号: US09187732B2公开(公告)日: 2015-11-17
- 发明人: Markus Wolschek , Andrej Egorov , Michael Bergmann , Thomas Muster , Christian Kittel
- 申请人: Baxter Healthcare SA
- 申请人地址: CH Opfikon
- 专利权人: Baxalta GmbH
- 当前专利权人: Baxalta GmbH
- 当前专利权人地址: CH Opfikon
- 代理机构: Loza & Loza, LLP
- 代理商 Michael Fedrick
- 优先权: EP07450176 20071005
- 主分类号: A61K39/00
- IPC分类号: A61K39/00 ; C07K14/005 ; C12N7/00 ; C12N15/86
摘要:
The present invention relates to a novel replication deficient influenza virus comprising a modified NS1 segment coding for a NS1 protein lacking a functional RNA binding domain and functional effector domain and having a heterologous sequence inserted between the splice donor site and the splice acceptor site of the NS gene segment. The virus can be used as vector for expression of various proteins like chemokines, cytokines or antigenic structures and to produce vaccines. A fusion peptide comprising part of the N-terminus of an NS1 protein and a signal sequence fused to the C-terminus of said NS1 peptide is also provided.