In an OCT system, the autocorrelation signal depends only on the strength of the signal in the object arm scattered back from a patient's retina and is a result of different reflections from different layers of the retina interfering with each other. The strength of the autocorrelation signal depends on how well focused the system is. Normally the autocorrelation signal is treated as noise. However by removing the reference path signal, the autocorrelation signal is easily measured and analyzed. The optimal focus can the then be found by adjusting the focus value until the autocorrelation signal is maximized.