发明授权
US09192631B2 Method for obtaining human microglial precursor cells from pluripotent stem cells
有权
从多能干细胞获得人类胶质细胞前体细胞的方法
- 专利标题: Method for obtaining human microglial precursor cells from pluripotent stem cells
- 专利标题(中): 从多能干细胞获得人类胶质细胞前体细胞的方法
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申请号: US13266845申请日: 2010-04-28
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公开(公告)号: US09192631B2公开(公告)日: 2015-11-24
- 发明人: Harald Neumann , Kristin Roy , Oliver Brüstle , Michael Peitz
- 申请人: Harald Neumann , Kristin Roy , Oliver Brüstle , Michael Peitz
- 申请人地址: DE Bonn
- 专利权人: Life & Brain GMBH
- 当前专利权人: Life & Brain GMBH
- 当前专利权人地址: DE Bonn
- 代理机构: Ohlandt, Greeley, Ruggiero & Perle, L.L.P.
- 优先权: EP09005880 20090428
- 国际申请: PCT/EP2010/055731 WO 20100428
- 国际公布: WO2010/125110 WO 20101104
- 主分类号: A61K35/30
- IPC分类号: A61K35/30 ; C12N5/0797 ; C12N5/079 ; A61K35/12
摘要:
A method for obtaining human microglial precursor cells, comprising: (a) providing a cell population comprising neural precursor cells, wherein the cell population is obtainable from embryoid bodies differentiated from human pluripotent stem cells; (b) differentiating the cell population comprising neural precursor cells into microglial precursor cells by culturing in medium comprising a growth factor selected from the group consisting of insulin and insulin-like growth factors; (c) expanding and enriching microglial precursor cells in medium comprising a growth factor selected from the group consisting of insulin and insulin-like growth factors and 10 to 150 ng/ml GM-CSF; and (d) isolating microglial precursor cells comprising CD45-positive cells.
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