A method of manufacturing an organic light emitting structure is provided as follows. A first electrode is formed on a lower substrate. A pixel defining layer is formed adjacent to the first electrode on the lower substrate. A preliminary charge transport layer is formed on the first electrode and the pixel defining layer. An organic light emitting layer is formed on the preliminary charge transport layer. The preliminary charge transport layer is selectively etched to form a charge transport layer. A second electrode is formed on the organic light emitting layer.