发明授权
- 专利标题： Synthetic siRNA detection method
- 专利标题（中）： 合成siRNA检测方法
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申请号： US13186941申请日： 2011-07-20
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公开(公告)号： US09200314B2公开(公告)日： 2015-12-01
- 发明人: Satoshi Yanagi , Eiji Kobayashi , Takashi Uemori , Hiroyuki Mukai
- 申请人： Satoshi Yanagi , Eiji Kobayashi , Takashi Uemori , Hiroyuki Mukai
- 申请人地址： JP Otsu-Shi, Shiga
- 专利权人： TAKARA BIO INC.
- 当前专利权人： TAKARA BIO INC.
- 当前专利权人地址： JP Otsu-Shi, Shiga
- 代理机构： Banner & Witcoff, Ltd.
- 优先权： JP2010-164109 20100721
- 主分类号： C12P19/34
- IPC分类号： C12P19/34 ; C12Q1/68 ; C12N15/10 ; C07H21/02 ; C07H21/04
摘要：
A method for accurately and easily detecting a synthetic siRNA, for example, a siRNA in which the 3′ end is DNA, and a kit used for the method are provided. The present invention relates to a method for detecting a siRNA in which the 3′ end is DNA, comprising: (a) adding polydeoxyadenosine to the 3′ DNA end of at least one strand of the siRNA to be detected to produce a polydeoxyadenosine-added RNA; (b) annealing a polydeoxythymidine primer having a tag sequence at its 5′ side to the polydeoxyadenosine-added RNA and synthesizing DNA from the primer by a reverse transcription; and (c) detecting the DNA synthesized in (b).
公开/授权文献
- US20120021420A1 Synthetic siRNA Detection Method 公开/授权日：2012-01-26
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