Systems and methods are provided that relate to frame formatting supporting mixed two and three dimensional video data communication. For example, frames in frame sequence(s) may be formatted to indicate that a first screen configuration is to be used for displaying first video content, that a second screen configuration is to be used for displaying second video content, and so on. The screen configurations may be different or the same. In another example, the frames in the frame sequence(s) may be formatted to indicate that the first video content is to be displayed at a first region of a screen, that the second video content is to be displayed at a second region of the screen, and so on. The regions of the screen may partially overlap, fully overlap, not overlap, be configured such that one or more regions are within one or more other regions, etc.