A channelization method of digital content and an audio-video server system are provided. A received operational behavior is packaged as an execution module, and the execution module is associated with a channel number. A correlation between the channel number and the execution module is recorded in a local channel list. Accordingly, when a switch command that contains the channel number is received, the execution module corresponding to the channel number is obtained from the local channel list, so as to perform the operational behavior to use the digital content.